We narrowed to 802 results for: eng

1. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

2. RNA Extraction Without A Kit

   Type

   Blog Post

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

6. Summer SciComm: Preprints

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   Blog Post

   ...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

7. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

8. Human Germline Editing Using CRISPR

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   Blog Post

   ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

10. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

11. Developing Lab Management Software for Biology

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    Blog Post

    ...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

12. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

13. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

14. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

16. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

18. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

19. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

20. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...