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We narrowed to 760 results for: mpo

Showing: 741 - 760 of 760 results

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ... similar experimental questions (see below). Importantly, although their role has changed, restriction...

  5. Optogenetics Guide

    Type
    Guide
    ...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...photoswitchable” proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...expression. These plasmids rely on the engineered two-component regulatory system YF1/FixJ. YF1 is a synthetic...desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185...K. 2011. Microbial opsins: a family of single-component tools for optical control of neural activity. ...

  8. Antibody Guide

    Type
    Guide
    ...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...no antibodies are available, change the least important factor (for instance, looking for a different ...

  12. Modular Cloning Guide

    Type
    Guide
    ...from different combinations of a common set of components (Weber et al., 2011). This method, based on Golden...transformation. Figure 1: Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. ...(Lohmann) with additional Level 0 modules and components to create plasmids with multiple transcriptional... multigene engineering, including CRISPR/Cas9 components. MoChlo: Modular Cloning Chloroplast Toolbox ...Arnold Driessen 96 plasmids (including CRISPR/Cas9 components) for expression or gene editing in filamentous...

  13. Chemogenetics Guide

    Type
    Guide
    ...DREADDs. Compound 21 has similar potency as CNO, while DCZ is more potent than either CNO or Compound 21. ...ligands can have high potency, which is potentially important for dosing in animal studies, made them attractive...demonstrating the need for alternative DREADD ligands. Compound 21, Deschloroclozapine (DCZ), Perlapine, and Olanzapine.... Both Compound 21 and DCZ seem to have minimal off-target activity, and do not seem to have the same ...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as...

  16. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... viral vector guide to learn about AAV vector components, production and common uses....bilayer surrounding the viral particle and are only composed of a protein capsid and the viral genetic material...packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table . Figure ...the blood to the CNS. Concatemer A DNA segment composed of multiple, repeated DNA sequences linked end-to-end...

  17. Molecular Cloning Techniques

    Type
    Guide
    ... and ligated into your desired plasmid. It is important to note that restriction enzyme target sites can...throughout endogenous DNA sequences. Thus, it is important to confirm that there are no additional sites ...replication process will repair the nicks. It is important to note that LIC has difficulty assembling DNA...

  18. Adenovirus Guide

    Type
    Guide
    ...vector guide to learn about adenoviral vector components, production and common uses....bilayer surrounding the viral particle and are only composed of a protein capsid and the viral genetic material...plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements table . ...

  19. Guide to Using Pooled Libraries

    Type
    Guide
    ...can help you begin to understand what genes are important to a certain phenotype, and allow you to design...only way to accurately evaluate which library components are influenced by selection, so it’s essential...

  20. Promoters

    Type
    Guide
    ...of a gene is initiated. Promoters are a vital component of expression vectors because they control the...encoding ribosomal RNA (rRNA), which is a main component of a cell’s ribosome structure. Ribosomes are ...
Showing: 741 - 760 of 760 results