We narrowed to 799 results for: Clu

...cloning by restriction enzyme digest (subcloning), including design and experimental procedures....Design (Choosing enzymes) Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency...handling, use proper personal protective equipment including laboratory coat, laboratory goggles, and gloves...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...

...multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175 flasks. Adjust...vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer or cell counter...

...of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent..., gentler practices, and colder reagents (not including the recovery media!). Do not proceed with Maxipreps...

...efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition...warranty of any kind either expressed or implied, including but not limited to the implied warranties of fitness...

...AATTAATT CCGCGCGG GTTAAC - 5' = 28 bp We also need to include additional bases to complement the overhangs generated...

...that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

...to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many different...

...Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more at (Link...

...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

...should also add a positive control (many companies include a positive control plasmid with their competent...

...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

...to each well (each well gets one dilution) Also include an untransduced well by using 150 μL of media in...

...others can interpret your images in the future. Conclusion Like any important laboratory instrument, you...

... discard into a biohazardous waste container. Conclusion Pipetting accurately and precisely is important...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency...

... to remove contaminants with lower densities, including empty capsids. The 60% step serves as a cushion...