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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 802 results for: Clu

Showing: 761 - 780 of 802 results

  1. Immunocytochemistry

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

  2. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

  5. Western Blot

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175 flasks. Adjust...vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer or cell counter...

  9. CRISPR Library Amplification

    Type
    Protocol
    ...of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent..., gentler practices, and colder reagents (not including the recovery media!). Do not proceed with Maxipreps...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition...warranty of any kind either expressed or implied, including but not limited to the implied warranties of fitness...

  13. Centrifugation

    Type
    Protocol
    ...to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many different...

  15. General Transfection

    Type
    Protocol
    ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

  17. Lentivirus Production

    Type
    Protocol
    ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

  18. Colony Formation Titering Assay

    Type
    Protocol
    ...to each well (each well gets one dilution) Also include an untransduced well by using 150 μL of media in...

  20. Pipetting Protocol

    Type
    Protocol
    ... discard into a biohazardous waste container. Conclusion Pipetting accurately and precisely is important...
Showing: 761 - 780 of 802 results