Tetracycline Inducible Expression
Type
Collection
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Immunology Research Plasmids and Resources
TypeCollection...have been grouped into tables based on the Gene Lists from Immport . Click the links below to go to the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Antibody Guide
TypeGuide...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated... -
Sequencing Primers
TypeGuide... along with a more complete list of common sequencing primers. These lists are provided for your reference...Find a list of universal sequencing primers and other common sequencing primers. Educational...sequence verification over the years. Below is a list of popular universal primers available from most...find your plasmid’s page and see what primers are listed under "5' sequencing primer" and "3' sequencing... -
Molecular Biology Reference
TypeGuide...the insert or other regions of the plasmid. For a list of commonly used primers, check out our sequencing...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...-related information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New ...Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK ... -
Modular Cloning Guide
TypeGuide...Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids to extend the MoClo-YTK (Dueber) for... Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing highly-tunable GPCR-based... standards. CyanoGate Kit Bacterial Expression Alistair McCormick Kit containing 96 parts and acceptor...Komagataeibacter Tool Kit (KTK) Bacterial Expression Tom Ellis DNA parts to create multigene constructs expressed... -
Optogenetics Guide
TypeGuide...relevant variants. This list is not exhaustive - please browse our curated list of opsins here to find ...depression, drug addiction, and more. Below is a list of commonly used microbial opsins, including a brief...Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale, genetically targeted optical control... -
Guide to Using Pooled Libraries
TypeGuide...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...cells Sequence those cells that pass (“win”) Get a list of genes involved in the selection NEGATIVE screen...between experimental and control cells Generate a list of gRNAs that disappear with addition of the selection... -
Educational Resources
TypeGuide...using Addgene’s materials, and career advice. Playlists include: Protocols Careers How-to Blog Read about... -
Molecular Cloning Techniques
TypeGuide...ends can still be fused in a seamless manner. To accomplish this, you can transform into yeast the fragments... -
Lentiviral Vector Guide
TypeGuide...PMID: 36348415 Stewart, S. A., Dykxhoorn, D. M., Palliser, D., Mizuno, H., Yu, E. Y., An, D. S., Sabatini...