We narrowed to 210 results for: cycs

1. Hot Plasmids and Viral Preps - September 2021

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   ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

2. Meet the New Addgene Board Members!

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   ...MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse,” Stegmeier...

3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   ... with a freezer wired to a charger and two deep cycle batteries for power so the samples could be kept...

4. Plasmids 101: TOPO Cloning

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   ... to include extra extension time after the last cycle of PCR to make sure that the "A" gets added to all...

5. New and Upcoming Viral Vectors - June 2019

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   ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

6. Scientists Map the SARS-CoV-2-Human Interaction Network

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   ...viruses rely on the help of human proteins in their lifecycle, identifying these potential human helpers of ...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ... blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research...

8. Viral Vectors 101: Viruses as Biological Tools

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   ...to produce more virus and continue the infection cycle. Depending on the type of virus used, the DNA (or...

9. A Quick Guide to a Career in Software Product Management

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   ... the users, gather more feedback, and start the cycle over again to refine the solution, or to come up...

10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired gene (whose...

11. 7 Tips to Secure a STEAM Internship This Summer

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    .... In order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility check...

12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent tumor growth through evasion of immunity...

13. Screening for Successful Genome Editing with Digital PCR

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    ...copies of the target DNA. Conventional thermal cycling is then performed to amplify the target using PCR...

14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    ... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...

15. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...OptoNBs. OptoNB coated beads were then imaged while cycling between dark and blue light which revealed a blue...

16. Sweating the Small Stuff: Details in the Lab

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    ... a chance to break what can be a rather vicious cycle.  Everybody’s Different   The truth is, there is...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...toward this goal for years as members of the ENCyclopedia of DNA Elements Project (ENCODE), an NIH-funded...

18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. Be quick at...

19. RaPID Detection of RNA-protein Interactions

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    ...untranslated regions (UTRs) to aid in the viral life cycle. Ramanathan et al. used RaPID to see what host proteins...

20. All in a Twist: dsRNA

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    ...acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed to reach...

21. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of...

22. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...light reduces the number of excitation/emission cycles per unit of time, decreasing the likelihood that...

23. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella’s autophagy-sensing abilities...

24. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes) step-function artificial anion...

25. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor growth, so it would...

26. CRISPRainbow and Genome Visualization

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    ... to track telomere dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system...

27. Antibodies 101: Stripping and Reprobing Western Blots

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    ...several times — some researchers report up to ten cycles! Just remember that a bit of sample is lost in ...

28. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

29. Adenoviral Vector Production and Troubleshooting

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    ... are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your primary low titer...

30. Rabies and Neuronal Tracing

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    ...Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...