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We narrowed to 910 results for: ire;

Showing: 91 - 120 of 910 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms and selection...Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic...

  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...acceptor, contains sequence that is over 80% GC. Some IRES sequences also contain regions that are GC rich.... refined and user friendly, to own the process entirely remains a large undertaking. Priority number one... usually confirm the insert through BLAST or by direct alignment to an NCBI reference sequence or a sequence...workflow and yield sufficient data to capture the entire viral genome. The full details of that workflow...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9...step 6 ). Select the clones identified with the desired deletion and move to larger plate or flask for ...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to...This plasmid also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde ...SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. Neuron. 2012...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction with FACS... the importance of the entire GFP protein, which is almost completely required to maintain fluorescent...co-factors, substrates, or enzymatic activity – it only requires the presence of oxygen during maturation. This...This meant that the protein could be taken directly from A. Victoria and expressed in any organism while...been done to expand the red-fluorescent protein repertoire; however, these proteins are unique from GFP ...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For...neutralization assays, part of a study that examined the repertoire of antibodies from 19 COVID-19 convalescent individuals... E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host...Harrison SC, Wesemann DR (2021) Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike ...

  8. Adenovirus Guide

    Type
    Guide
    ...different purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP ...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors...got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4, E2a and... available inside of the adenoviral capsid and directly correlates to the size of the DNA removed from... of genetic cargo. Production of HCAdV vectors requires using an additional adenoviral helper virus (HV...system, recombination between both plasmids occurs directly in the HEK293 packaging cell line, engineered ...of the genome adjacent to the left ITR that is required for viral genome packaging. In helper viruses,...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances the efficiency of homologous...applications that require simultaneous modification of all alleles of a gene, or which require modifying multiple...experiment. For this reason, the AAV-based approach requires G418 selection of infected clones, making it unsuitable...suited for tissue culture based approaches that require modification of only a single allele of a gene,...deliver large repair templates for applications that require large gene fusions. The current state-of-the-art...PMCID: PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated virus for enhanced...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into...booster cassette), their expression heterogeneity required single cell screening to isolate a clonal cell...producing ultrasound contrast, and gene silencing required addition of chromatic-opening agents to boost ...contrast. It turned out that Anabaena mARGs did not require a booster cassette, either, allowing us to reduce...marked with Emerald GFP. These plasmids can be used directly in transient transfection in rtTA+ cell lines ...

  11. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of...

  13. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR Activate and Interfere technologies also indirectly modify local chromatin state. Want more information...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it...

  18. Plasmid Collections

    Type
    Collection
    ... are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...

  19. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created...

  23. AAV Packaged on Request

    Type
    Collection
    ...needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities....

  24. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...emission of a photon, the acceptor molecule is not required to be fluorescent. A variety of organic dyes ...

  25. Zebrafish Plasmid Collection

    Type
    Collection
    ...Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein abundance, size, and ...

  26. Open Enzyme Collection

    Type
    Collection
    ... new functions through genetic engineering and directed evolution. More information about the collection...

  28. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... fluorescent protein downstream, separated by an IRES element. You did a lot of work! But let’s take a...-thi-1 or thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption...leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation...mutation in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation ...This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13 mcrB ...

  29. Lentivirus Plasmids

    Type
    Collection
    ...lentivirus: these viruses are based on HIV-1, which may require additional lab biosafety procedures. Additionally...
Showing: 91 - 120 of 910 results