...allow researchers to avoid the off-target effects of hi-multiplexing within in a cell, while potentially ...

...cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

...our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

... At the Broad, my colleague Cory Johannessen and his team have recently demonstrated the power of this...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

...as well as cell lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using...

...Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...

...start site in those 15,977 human genes. Weissman and his colleagues demonstrated in a paper in Cell that they...

...Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also began...

...sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-propagating... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

...Allinen et al., 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449...

...them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

...paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ...

...this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...

...speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded...

...this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that...

...John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid...

...-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells)  that allow for continuous expression of...

...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which...

...I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can...

... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential...

... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to...

... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

...our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

...al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this...

...developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are...

...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing...

...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the...

... so as to benefit all people and the planet. For his many efforts in open science, Endy was recognized...

...created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be ...

...Big thank you to Jessica Welch, Mike Lemieux (for his advice and for modeling in the picture above), and...

...mauvine, was discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority...

...ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions...bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,” ChIP-... of modified histones, a genomic region that has mono-methylated and acetylated histones, but not tri-...tri-methylated histones, functions as an enhancer. We can therefore denote enhancers as follows: (mono_methylation...of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations of the amino ...combination of coiled DNA and structural proteins called histones. Chromatin folds into secondary structures (loops...

...AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels around Europe...and money but cells lose provenance (this is the history of the cell line consisting of records of the origin...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

...expression of the HIS3 gene, which is required for histidine manufacture and therefore growth on his-deficient...that repress instead of activate transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait... is an example of the former. In this system, histidine-auxotrophic host strains are used to select for...

.../threonine kinases implicated in human diseases. His spare time is spent trying to raise the alpha isoform...

...enthusiastically foster independence Your partner can make his or her own dinner if you are going away to a conference...

...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

...Tabin’s lab who took inspiration from a comic book his daughter was reading. SHH is involved in the hedgehog...bagpipe mutants vaguely resemble a bagpipe and historically, bagpipes were made using the stomachs of different...

...Comprehensive Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...

... Research, Ludwig-Maximilians-University Munich. His lab develops iPSC-based models of neurodegenerative...

...frequent bouts with Impostor Syndrome, Joe has found his people through meetings and social media. Joe is ..., and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual... career scientists, students, and those from historically underrepresented groups. Through such mentoring...

...reading more about the research is of interest. In his research, Scott Spicer concludes that “… the emergence...

...but it wasn’t until one of our blog writers posted his article (Developing Lab Management Software for Biology...

...skills and research independence needed to pursue his or her chosen career path,” but individual institutions...

...Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 ... Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

...blogger Jacob Lazarus from Harvard. Jake received his MD and a PhD in Cell and Molecular Biology from the...

...to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based strategy...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...