We narrowed to 1,013 results for: CAL

...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts (Link opens...biology describes the rational engineering of biological systems for novel functions. Addgene is working...

...while the wet-lab resources, like constructs or biological material, can be deposited in the specific repositories...

...libraries to screen for genes involved in specific biological processes. For more information, see our Guide...pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...

...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910...pAAV-TBG-ER-TurboID TBG-driven, constitutive Expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long ... Cre-dependent Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long ...

...an experimental system comes with fewer ethical and logistical challenges than does working with animals...grown to include local school districts, professional education associations, and local organizations such...Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant...University. Established in 1991, the Arabidopsis Biological Resource Center (ABRC) is one of two global stock...preference for using animal systems to demonstrate biological concepts - and you have yourself a plant publicity...publicity crisis. The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment...conducting teacher workshops, or staffing a booth at local science festivals, ABRC gets the word out about ...

...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ...Zhang’s lab describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...distinct effector domains that work together synergistically, modeled after natural transcription activation...GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang lab's Nature ... is robust, specific, and can facilitate genome-scale gain-of-function screening when combined with a ...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target...

...novel virus strain from the Coronaviridae family called SARS-CoV-2. In 2020, scientists worked at lightning... enzyme that cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains...Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor . Cell, 181 (2), 271...

...Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins...Geraldine Seydoux's lab has developed a systematic and scalable method to create marker-free mutations, insertions...co-integrated markers or footprints, and can be scaled up for systematic editing of multiple genes. Plasmids...

...collection of CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation...sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology...all-around platform for CRISPR knock-in design at scale. It uses an open-source algorithm to automate and...

...specific and much faster than any genetic or pharmacological manipulations on cells, and has potential use... platform for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to...set of tools that can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...platform at Addgene. Braselmann E, et al. Nature Chemical Biology. 2018 PubMed PMID: 30061719. New tools...expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018. PubMed: PMID 30220597. ACE system...

...pH-stable OFP (Antares), and Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...

... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem...PubMed . Return to GeCKO Library SAM Library Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

...reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein...intracellular calcium levels within the cell. When levels of Ca2+ are high in the cell, calcineurin dephosphorylates...attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve...identify new antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial...screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical issues, ...genetic tool for labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...

...Catalytically inactive CRISPR plasmids for purification of target loci....DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA...

...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise ...several separate Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for a ntiviral...

...or whole animals presents unique challenges. Genetically-encoded fluorescent proteins most suitable for... Backbones Fluorescent Proteins: Subcellular Localization Luciferase Plasmid Collection Viral Vector Packaging...

...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced...sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through...

...Molecular mechanisms and biological functions of siRNA . International Journal of Biomedical Science, 13 (2), ...

...also available as 20 µL. The 20 µL option is an economical choice that can be used to validate the performance...performance of various serotypes before conducting larger scale experiments. For more information, see our full ...

... non-dividing cells, and relative ease of large-scale production. In order to produce recombinant adenoviral...Protocols General Transfection Bert Vogelstein’s lab practical guide for using the AdEasy™ System Content last...