We narrowed to 775 results for: Mos

...Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used in research...

...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

...Phosphorylation Protein phosphorylation is one of the most abundant forms of posttranslational modification...

...sites, and catalyzes recombination between them. Most SSR enzymes are categorized into two distinct families...

...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...

...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

... can be measured by the luminescence output. The most popular types of luciferase found in Addgene's collection...

...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...

...different cell types and cloned into an AAV backbone. Most enhancers were derived from human, mouse, and macaque...

...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...

...green are the ones loaded onto the qPCR plate for most samples. If sample is expected to have a titer <...have some small amount of background signal that is most evident during initial PCR cycles. This background...

...Although our recommended temperature should kill most potential contaminants, certain types of spores ... agar will remain liquid at this temperature but most antibiotics will not break down at this temperature...

...is. While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone...