... following intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F...

...Phosphoprotein Reagents Protein phosphorylation is one of the most abundant forms of posttranslational modifications...

...labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that...

...Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used in research...

...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

...appropriate. If your gene of interest should be mostly inactive, and only turned on occasionally, using...

...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

...TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA prothymosin alpha a TAL3344 & TAL3345 TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA...

...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...

...number of cells is limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to...

...gene in vitro and in vivo to use in biosensors. The most common types of luciferase found in Addgene’s collection...

...different cell types and cloned into an AAV backbone. Most enhancers were derived from human, mouse, and macaque...

...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...