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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 771 results for: Mos

Showing: 61 - 80 of 771 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that...

  2. Lentivirus Plasmids

    Type
    Collection
    ...Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used in research...

  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

  6. Using a Light Microscope Protocol

    Type
    Protocol
    ...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

  8. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA prothymosin alpha a TAL3344 & TAL3345 TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ... can be measured by the luminescence output. The most popular types of luciferase found in Addgene's collection...

  10. Caltech Systemic Capsids

    Type
    Collection
    ...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...number of cells is limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to...

  14. Chemogenetics Guide

    Type
    Guide
    ...Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender U (2025)...Gorantla N, Simkins J, Crespo EL, Ikefuama EC, Tree MO, Prakash M, Björefeldt A, Barnett LM, Lambert GG,...Addgene's collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic...Early Chemogenetic Receptors: RASSLs DREADDs PSAMs LMOs Planning Your Experiment References Chemogenetic... channels targeted to specific cell populations (most often neurons) that can be turned on or off by the...have few, if any off-target effects. They are the most widely employed type of chemogenetic receptors. ...: hM1Dq, hM3Dq, and hM5Dq, however, hM3Dq is the most widely employed. Gi-DREADDs signal through the Gαi...

  15. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

  16. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

  17. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

  18. Centrifugation

    Type
    Protocol
    ...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

  20. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...
Showing: 61 - 80 of 771 results