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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 550 results for: NOL

Showing: 61 - 80 of 550 results

  2. CRISPR References and Information

    Type
    Collection
    ...genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole Shirley Liu Lab . For...

  3. Brain Armamentarium

    Type
    Collection
    ...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

  4. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Sterilize your lab bench by spraying it down with 70% ethanol and wiping it down with a paper towel. Maintain...

  6. Immunocytochemistry

    Type
    Protocol
    ... for all. Alternative fixation methods such as methanol or acetone may be better for some applications...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...working flame. Spray down the bench with a 70% ethanol solution and wipe down with a paper towel. Count...

  9. Brain Initiative Collection

    Type
    Collection
    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

  10. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250 U/µl, Millipore #71205...

  11. Antibody Guide

    Type
    Collection
    ...Flow cytometry Flow cytometry, used commonly in immunology, allows you to identify, analyze, capture, and...

  13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

  14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... AAV purification with iodixanol gradient ultrafugation Overview: Iodixanol gradient ultracentrifugation...brief overview of how iodixanol purifications works, shows how to create the iodixanol gradient layers, and...master iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers...feel for layering the iodixanol solutions and let me double-check that the iodixanol gradient solutions ...and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the virus particles...then treated with benzonase immediately before iodixanol purification. This approach gives me a bit of ...uses a gradient of different concentrations of iodixanol to separate out contaminants from an impure AAV...

  15. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays...point that people cannot imagine any use for GM technology other than to sustain those techniques. We feel... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...

  16. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...sample. Ethanol carry-over from purification protocols is also a common concern, and while ethanol can reduce...residual guanidine thiocyanate. Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and...experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own characteristic...ones most likely to impact absorbance readings are phenol, guanidine salts, and detergents (Figure 1C-D; ...means that even samples with quite high levels of phenol (up to 600 ppm according to Loughrey and Matlock...yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's...towards 270 nm. If your purification protocol uses phenol, then it is important to confirm that your sample...

  17. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...that had Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success ...biological questions, you need a tremendous amount of technology development alongside it.” Doench starting working...Perturbation Platform and encompasses a variety of technologies to functionally investigate the human genome...genome. Doench says he enjoys working on technology development under the Platform model at the Broad Institute...Institute because they are “not just developing technology for its own sake,” but work in true collaboration...history of RNAi left him a bit skeptical of the technology at the start of the CRISPR craze, but he says...justified… it has been just an incredibly useful technology.” He goes on to explain that, “everything that...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    ...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...perusing classic papers or are brainstorming new technological innovations. Let’s take a look at three historical... use of Sanger sequencing. Building upon this technology, radiolabelled nucleotides were replaced with... DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it...properties of nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively... is projected that nanopore-based sequencing technologies could one day sequence an entire genome for ...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer...is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot on what...Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA... Created with BioRender.com. Two newer technologies offer an alternative to HDR for introducing ... another target C or A in the window. Another technology, prime editing (reviewed in Anzalone et al., ... although these are still early days for this technology, and the user may need to optimize numerous parameters...CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...please read on to learn more about this exciting technology from the folks at Transposagen. This post was...engineering approaches. Most importantly for this technology, however, is that the transposase also enables... with no known upper limit [1]. The piggyBac™ technology can be used for numerous applications, including...of interest. Fortunately, by combing nuclease technology with the piggyBac™ system, these challenges can... DNA using the piggyBac™ transposon selection technology using a site-specific nuclease to stimulate homologous...of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette. While...summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research applications...
Showing: 61 - 80 of 550 results