We narrowed to 94 results for: SAB

1. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...DNA. Zeocin is an all-in-one antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and...

2. CRISPR 101: Making a Knock-In Cell Line

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   ...can try one or more of the below suggestions. Disable the other repair mechanisms Mammalian cells have...

3. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...through a nerve wracking commute complete with a disabled train, and now you’re sitting in the lobby of ...

4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   ...synthesis Before you start your cloning, it is advisable to remove unwanted type IIS restriction sites ...

5. Designing Your Chalk Talk for the Academic Job Interview

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   ...of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want and you...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...various research applications, they have become indispensable tools for manipulating cells and organisms. ...

7. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...23371551. PubMed Central PMCID: PMC3916822. Schopp, Isabel Myriam, et al. "Split-BioID a conditional proteomics...

8. Technique: Probe Phage Genomes for Host Binding Proteins

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   ...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

9. Finding Your Science Policy Path

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   ...STPF cohort members came to DC to do either a sabbatical year or transition away from academia as tenured...

10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...patterns of spread from the injection site it is advisable to try a few different injection titers and volumes...

11. Important Considerations When Using AAVs

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    .... However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~2.3 kB [9])....

12. CRISPR Plasmids - Empty gRNA Vectors

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    ...Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian/Lentiviral...Mammalian/Lentiviral hU6 none S. pyogenes Puro Sabatini sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) 71485 Mammalian...

13. CRISPR Guide

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    ...them referred to as INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting... 661–667. PMID: 25961408 Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014). Genetic screens...Livny, J., Regev, A., Koonin, E. V., Hung, D. T., Sabeti, P. C., Collins, J. J., & Zhang, F. (2017). Nucleic...

14. Custom CRISPR Screens & the Green Listed Software

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    ... (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference...

15. Plasmids 101: Modular Cloning Applications and Kits

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    ...For many MoClo systems, the individual parts are reusable, saving time and money on future assemblies. If...

16. Live and Let Dye: Self-Labeling Protein Tags

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    ....1016/j.chembiol.2019.01.003  Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). ...

17. Delivery Methods for Generating iPSCs

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    ...not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is ...

18. CRISPR 101: Cytosine and Adenine Base Editors

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    ...position upstream of the PAM Kim et al., 2017 SaBE3, BE3 PAM variants, BE3 editing window variants   ...

19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    ... lab's system called INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) and...

20. Using AAV for Neuronal Tracing

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    ...Miyamichi, K., Akhtar, N., Luo, L., Whelan, S.P., Sabatini, B., and Cepko, C.L. (2011). Anterograde or retrograde...