We narrowed to 92 results for: SAB

1. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   ...the particular features of your plasmid, it is advisable to check out the genotype of your E. coli to ensure...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...for the first time, you've got a collection of reusable, off-the-shelf genetic elements that allow you...

3. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ...through a nerve wracking commute complete with a disabled train, and now you’re sitting in the lobby of ...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...DNA. Zeocin is an all-in-one antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and...

5. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...can try one or more of the below suggestions. Disable the other repair mechanisms Mammalian cells have...

6. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want and you...

7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...various research applications, they have become indispensable tools for manipulating cells and organisms. ...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...23371551. PubMed Central PMCID: PMC3916822. Schopp, Isabel Myriam, et al. "Split-BioID a conditional proteomics...

9. Zinc Finger Consortium: OPEN Reagents

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   Collection

   ...Strain CSH100 Content blocked, you may need to disable your ad-blocker....

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ...A., Horwitz, N., Beggs, A. H., Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of...

11. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

12. Finding Your Science Policy Path

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    Blog Post

    ...STPF cohort members came to DC to do either a sabbatical year or transition away from academia as tenured...

13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...patterns of spread from the injection site it is advisable to try a few different injection titers and volumes...

14. Important Considerations When Using AAVs

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    Blog Post

    .... However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~2.3 kB [9])....

15. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). ...

16. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...For many MoClo systems, the individual parts are reusable, saving time and money on future assemblies. If...

17. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ... (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference...

18. Delivery Methods for Generating iPSCs

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    Blog Post

    ...not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is ...

19. CRISPR References and Information

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    Collection

    ...Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA...

20. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post

    ... lab's system called INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) and...