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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,036 results for: STI

Showing: 61 - 80 of 1036 results

  1. Genetic Code Expansion

    Type
    Collection
    ...GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community ...non-canonical amino acid can be incorporated using the existing protein translation machinery. To expand the genetic...a protein so that it is responsive to specific stimuli, such as light. On a broader scale, the expanded...made when the ncAA is included. For any and all questions you have while designing, implementing, or troubleshooting...127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted phenylalanine derivatives and tyrosinyl ethers...pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine analogs Mammalian TAG Huiwang Ai...

  2. Antibody Production

    Type
    Collection
    ... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm..., freezing at -80 °C, thawing on ice, and then testing for loss of potency against a non-treated control...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However, Addgene...

  3. Distribution to Industry

    Type
    Collection
    ...HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity. These are grouped...easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple...researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in ...:1479-91. PMID: 24360272 Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg.... Science . 337(6096):816-21. PMID: 22745249 Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT...effects. Nature . 529(7587):490-5. PMID: 26735016 Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng...

  5. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64...vector, CMV promoter, Hygro Campeau Viral Service Suggestions Don’t see what you are looking for? Please let...viral format by filling out our Viral Service Suggestion Form (Link opens in a new window) . Please note...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...resource center based in China focused on collecting existing zebrafish resources and developing new lines and...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should be added to this...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant...check for the presence of particles still in solution. Continue to stir until all particles have dissolved...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier...following resources may be of interest for groups interesting in implementing intersectional experimental design... in a new window) for the molecular design and testing of novel INTSRECT plasmids for groups interested...Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the...

  9. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...phenotype. The National Human Genome Research Institute (NHGRI) is funding this collaborative program ...program that brings together teams of investigators who will use state-of-the-art experimental and computational... funded by the National Human Genome Research Institute (NHGRI) (Link opens in a new window) . Find IGVF...

  10. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... of the primer assist with restriction enzyme digestion (usually 3-6bp). Restriction Site: Your chosen...and then TAAGCA to improve restriction enzyme digestion. This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA... You can generate the reverse-complement using existing software (a quick internet search will lead you... The PCR product is now ready for restriction digestion. Digest Your DNA Set up restriction digests for...have compatible overhangs or no overhangs after digestion, you will need to use a phosphatase to prevent...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...purification. After purifying the DNA , conduct a diagnostic restriction digest of 100-300ng of your purified...

  11. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...teaching different types of courses, at any type of institution, and at different points in their careers to ...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...

  12. mTOR Pathway

    Type
    Collection
    ...Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is...known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53...nrd4505. PubMed PMID: 25743081 . Do you have suggestions for other plasmids that should be added to this...

  13. Microbiology Resources

    Type
    Collection
    ...plasmids Addgene distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases...Synechococcus sp. Thermus sp. Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids...community by depositing your useful tools , or by suggesting plasmids for the collection. Return to top Plasmid...opens in a new window) Return to top Do you have suggestions for other plasmids that should be added to this...

  14. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...called "sticky ends", are what allow the vector and insert to bind to each other. When the sticky ends ...depicts the ligation of two sticky ends that were generated by EcoRI digestion: Usually, scientists select...that have been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically...ligating to itself during the ligation process. If the sticky ends on either side of the vector are compatible...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ...years from symptom onset. Disease mechanisms are still poorly understood but several genes and mapped loci...other large scale studies. Researchers are now investigating the role that these additional genes may play...Noteworthy: Study aberrant axon initial segment (AIS) plasticity with a motor neuron-specific reporter and a PAX7...accelerate discovery and advance development of diagnostics and treatments for Alzheimer’s disease and related...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...sgRNA(F+E) 59986 Other/Ciona intestinalis BsaI none S. pyogenes Christiaen p(U6a-BsaI-gRNA) 65955 Other...Golden Gate cloning or the Gibson Assembly. Chen Boosting CRISPR/Cas9 multiplex editing capability with ...into Tol2-based pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen...genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that should be added to this...

  17. Video Library

    Type
    Protocol
    ...Protocol Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction...free to email us at [email protected] with any questions. Protocols Video protocol guides for standard ...- Requests Your guide to the MTA process when requesting samples from Addgene Technology Transfer How ...Ordering Page MTA FAQs Answers to frequently asked questions about the MTA process Help Center How to Deposit...

  18. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... with the recipient plasmid's MCS. However, you still need to avoid restriction enzymes that cut within...have compatible overhangs or no overhangs after digestion, you will need to use a phosphatase to prevent...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...purification. After purifying the DNA , conduct a diagnostic restriction digest of 100-300ng of your purified...orientation of your insert, so you may want to design a diagnostic digest for this purpose. Congratulations, you...

  19. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ... using modular components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids...Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids GFP GFP-dependent transcription factors...activity Michael Lin See Plasmids Do you have suggestions for other plasmids that should be added to this...

  20. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...gel from the plastic wrapper and rinse with deionized water. Gently remove the white sticker on the bottom...Rocking platform Fume hood Metal spatula Razor blade Plastic tray Gel imaging system ImageJ or similar software...Section 2: Staining the Gel Place the gel in a plastic tray with 100 mL of deionized water. Rinse gel ...the standard curve is y = 14725x + 3748.4, then substitute 18179.442 for y and solve for x. 18179.442 = ...
Showing: 61 - 80 of 1036 results