Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 955 results for: Tes

Showing: 61 - 80 of 955 results

  1. Rett Syndrome

    Type
    Collection
    ...window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms are reversible in...Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

  3. Retrograde AAV viral preps

    Type
    Collection
    ...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ...tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence of tetracycline...

  5. DNA Purification Without a Kit

    Type
    Blog Post
    ...DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for use! Plasmid...centrifugation and washed before DNA is eluted with water or TE. The Bishop Lab’s glass milk protocol is adapted ...than 30 minutes and only requires incubating the columns with 1M phosphoric acid for 3 minutes followed...syringe-based method can be completed in 20 to 30 minutes. Additionally, while commercial plasmid prep kits...same as those provided with the kit. Although not tested by the authors, it’s possible that used plasmid... the National Academy of Sciences of the United States of America, 76 2, 615-9 . https://doi.org/10.1073...

  6. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100%...100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized...the column using water or a neutral buffer such as TE. You will now have plasmid DNA that has been purified... Water-saturated phenol-chloroform can be used if TE-saturated is not available. Vortex microfuge tube... paper towel for 5-20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension...min. Centrifuge the tube for 5 min at 12,000 g. Notes: Pellet contains proteins, cell fragments, salt ...

  7. Neuroscience

    Type
    Collection
    ... represent the official views of the National Institutes of Health....

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

  10. Antibodies

    Type
    Collection
    ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

  11. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

  19. Mammalian RNAi Tools

    Type
    Collection
    ...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...
Showing: 61 - 80 of 955 results