We narrowed to 760 results for: Vars

...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors...

...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified...

...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

... of their low immunogenicity. Viral vectors also vary in the composition of their viral genome, expression...

...are engineering zinc finger arrays targeted to various zebrafish genes. The table below lists zebrafish...

... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...

...Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization...

... E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms. See...

...viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific...

...Jonathan Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System...

... used for AAV vector production suitable for a variety of serotypes and applications. Read our AAV vector...

...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

...all cells and all tissues. Ras can activate a variety of pathways, which ultimately turn on genes involved...

...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

...Toolkit) (Link opens in a new window) A library of various python functions for the purpose of designing and...

...D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier CE, Tipton GJ...

...several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive...DNMT1 Hereditary sensory neuropathy type IE Paul Khavari 25044 pDEST53-LRRK2-WT LRRK2 GFP CMV Parkinson'...pCB268 APP Flag, HA, Myc CMV Alzheimer's Alexander Varshavsky 53810 SYNJ1-145_pCSdest SYNJ1 CMV, SP6 Parkinsonism...Hereditary diffuse leukoencephalopathy Markku Varjosalo 187827 pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV...

...Swanson, M. I., Tam, B., Ticoll, A., Turner, J. L., Varhol, R., Vermeulen, J., Watkins, R. F., Wilson, G.,...

...science. General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide...buffer. Pro-Tips The volume of lysis buffer will vary depending on the size of your cell pellet but will...between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location of the protein...sample. Pro-Tip The ideal total protein loaded will vary between samples and target proteins but is typically... 1X TBST. Pro-Tip The ideal blocking buffer will vary between antibodies. Refer to the manufacturer's ...blocking buffer. Pro-Tip The optimal concentration will vary between antibodies but is typically between 1–10...

...increases reproducibility, as it eliminates the variation associated with repeated transient transfection...lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers to find one suitable...transduction efficiency. Infection efficiency will vary between cell lines. It is not recommended that lentiviral...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...transgene has integrated in different locations in the various cells in the culture. This is because lentiviral...of the lentivirus used, cells may also exhibit varying numbers of integration events. In other words, ... transgene in their genome. This can result in varying expression levels of the transgene from different...