...have deposited plasmids include: Joan Brugge , Harvard Medical School Klaus Hahn , UNC Richard Hynes ,...

...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

...additional tools are being developed to regulate a variety of other activities. By using GFP as a scaffold...

...Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger...

...developed for 293T cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended to subject...

...comprehensive collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs...

... scientists. Additionally, we have developed a variety of educational resources to help you choose and...

...Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal ...

... enzymes, allowing scientists to target a wide variety of recognition sequences. For a list of many commonly... 1 µg of DNA. The total reaction volume usually varies from 10-50 µL depending on application and is largely...is due to "Star Activity" and can happen for a variety of reasons, including high glycerol concentration...

...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI depending on the user, ...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 days after transfection...

...chromatin state. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...AAV particles. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...repair (HDR). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...developed for A549 cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended that lentiviral...

...fused to dCas9 or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, PA, and biotin...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...

...efficiency than NHEJ. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter...