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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 771 results for: Vars;

Showing: 61 - 80 of 771 results

  1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... enzymes, allowing scientists to target a wide variety of recognition sequences. For a list of many commonly... 1 µg of DNA. The total reaction volume usually varies from 10-50 µL depending on application and is largely...is due to "Star Activity" and can happen for a variety of reasons, including high glycerol concentration...

  2. General Transfection

    Type
    Protocol
    ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI depending on the user, ...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 days after transfection...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Enhancers were evaluated in mouse brains, using various delivery methods including intracerebroventricular...contains best-in-class AAV plasmids to target a variety of cell populations in different brain regions....

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...developed for A549 cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended that lentiviral...

  6. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also... plating on the appropriate antibiotic and try varying the amount of recipient plasmid to insert in the...

  7. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...fluctuations and to minimize well-to-well variability that result from a variety of causes, such as pipetting error...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and forth...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...P8833 Target cells Varies based on your experiment Culture media for target cells Varies based on your experiment...to 20 μl serum-free OPTI-MEM TIP: You may want to vary the ratio of shRNA plasmid, packaging plasmid, and...experiment Materials for assay Varies based on your experiment Detailed protocols for preparing polybrene...

  9. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based on DNA...for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It is also...your transformation worked. You could also try varying the amount of recipient plasmid to insert. Isolate...

  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...optimal concentration of primary antibody to use will vary and needs to be empirically determined. If possible...buffer. Pro-Tip The ideal antibody concentration will vary and needs to be empirically determined. We suggest...concentrations. The ideal range of the standard curve will vary between targets and needs to be empirically determined...

  11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...results in a heterogenous polyclonal population that varies in the number of integration events and the site...culture has, in theory, been transduced. There are various schools of thought on using selective medium throughout...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

  12. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...vector DNA and insert DNA used in the ligation will vary depending on the size of each and their concentration...failed ligations. The following table indicates the various controls: Control Ligase Interpretation Uncut vector...

  13. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...recombinant antibody can be purified for use in a variety of applications. Sharing speeds science. We believe...to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should be empirically determined...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...protocol. Sample Data Figure 1: HEK293T cells at various confluencies. This AAV production protocol should...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637 NS2B C 79636... activity have remained challenging due to the variable availability of enhancer sequences in different...the entire genome for genes that regulate a wide variety of phenotypes, including but not limited to cell...CIDAR MoClo) Parts Kit. This Kit consists of a variety of plasmids containing many different promoters...RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined with your...creation of many E. coli constructs with greatly varied levels of expression. The 93 plasmids in the CIDAR...gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts...

  19. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger...
Showing: 61 - 80 of 771 results