We narrowed to 125 results for: actin

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

   ...fact they are just non-specific or indirectly interacting. The CUT&RUN method Enter CUT&RUN, an enzyme-based...

2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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   Blog Post

   ...person for my department.” Caitlin does this by acting as a facilitator between her team, the sales department...

3. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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   ...appointments with people all over the world. Think about contacting alumni of your lab or university or use your ...

4. Antibodies 101: Epitope Availability

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   ...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

5. In Vivo Biotinylation of Bacterial Fusion Proteins

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   Blog Post

   ... calculate the amount of bound ligand (after subtracting out non-specific binding). Historically, glutathione-S-transferase...

6. Fluorescent Biosensors for Measuring Autophagic Flux

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   ... glycine deletion, so it remains in the cytosol acting as an internal control. Autophagic flux is estimated...

7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   ...the trip. Preparations ahead of time included contacting YNP staff to arrange permits to visit places ...

8. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...the ITRs and initiate replication. ITRs are cis-acting elements, which means that they are in the same...

9. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...a good fit for you if you are energized from interacting with others, and have strong communication skills...

10. GCE4All: Making Genetic Code Expansion Accessible

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    ...specific to the ncAA and orthogonal to (i.e. non-interacting with) the cells’ own natural translation machinery...

11. Antibodies 101: ChIP

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    ... focuses on the DNA your protein is actively interacting with, reducing background information. Your options...

12. Antibodies 101: Buffers, Storage, and Conjugates

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    ...conjugates are fluorophores, which work not by reacting to a chemical, but by emitting a specific wavelength...

13. OCaMP: A New Calcium Indicator for Neural Imaging

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    ...limited quantity of AAVs that can be requested by contacting Alexander Lohman ([email protected]).  ...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...means you aren’t getting any background or cross-reacting. We’ve developed this for transcription factors...

15. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists...

16. RaPID Detection of RNA-protein Interactions

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    ...requires 16-18 hours to sufficiently biotinylate interacting proteins, which results in poor spatiotemporal...

17. Transferable Skills Guide: Public Speaking

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    ...doesn’t know the term “diving board” so you end up acting it out in front of a very confused group of visiting...

18. All in a Twist: dsRNA

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    ...handle most of these intermediates, with each one acting on specific substrates and in distinct cell cycles...

19. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...isolate an antibody’s immune complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). ...

20. Transferable Skills Guide: Problem Solving

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    ... description, but this skill is not just about reacting to technical issues. This skill also comes into...