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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 565 results for: nol

Showing: 61 - 80 of 565 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...viruses containing transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP Shuttle...

  2. Retrovirus Plasmids

    Type
    Collection
    ...-G recommended for the FELIX (FIV-based) system Nolan 35617 pCAG-Eco Envelope Ecotropic MLV expressing...

  3. Worm Expression Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids...

  5. Viral Production

    Type
    Collection
    ...helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation and concentrated ...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ...working flame. Spray down the bench with a 70% ethanol solution and wipe down with a paper towel. Count...

  7. Brain Armamentarium

    Type
    Collection
    ...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...

  11. COVID-19 Resources

    Type
    Collection
    ...list of cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid...

  12. CRISPR References and Information

    Type
    Collection
    ...genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole Shirley Liu Lab . For...

  13. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250 U/µl, Millipore #71205...

  15. Brain Initiative Collection

    Type
    Collection
    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

  17. Antibody Guide

    Type
    Collection
    ...Flow cytometry Flow cytometry, used commonly in immunology, allows you to identify, analyze, capture, and...

  18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... AAV purification with iodixanol gradient ultrafugation Overview: Iodixanol gradient ultracentrifugation...brief overview of how iodixanol purifications works, shows how to create the iodixanol gradient layers, and...master iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers...feel for layering the iodixanol solutions and let me double-check that the iodixanol gradient solutions ...and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the virus particles...then treated with benzonase immediately before iodixanol purification. This approach gives me a bit of ...uses a gradient of different concentrations of iodixanol to separate out contaminants from an impure AAV...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays...point that people cannot imagine any use for GM technology other than to sustain those techniques. We feel... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...
Showing: 61 - 80 of 565 results