We narrowed to 883 results for: prot

... remote learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors...their curriculum. From the details about personal protective equipment to learning about how to use a tissue...learn the ropes! All material can be found on our Protocols page and YouTube page.  Happy learning, and welcome...

...with permission.   Mutations in the spike protein are one of the key drivers in determining whether...the current vaccines are effective or not. This protein both elicits an immune response as the target of...

...started on our deposit page. Protocols.io Website: http://protocols.io/ Protocols.io is the creation of ZappyLabs...resource. Nature Protocol Exchange Website: http://www.nature.com/protocolexchange/ Nature Protocol Exchange ...biomedical topics. Protocol Online Website: http://www.protocol-online.org/ Protocol Online is one of the...the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since ...popularity, I decided this protocol would be a worthy first contribution to the protocols.io community. Did I mention... updated protocols So that’s great, but what if someone in China improved upon that protocol? How would...comment, annotate, and edit protocols. After my introduction to protocols.io, I decided to research what...

...controls the activity of a microtubule-associated protein to regulate cell growth dynamics Article contributed...normally sits on microtubules to attract other proteins involved in their growth and extension. Now, they... domains. When both halves of the modified EB1 protein are overexpressed in cells, microtubule growth ...conformational changes, and as a consequence, EB1 protein function is inactivated by the separation of its...cellular dynamics. The photoinducible control of protein function is more specific and much faster than ...fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo...on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance and live ...

...to change protein levels? Until recently, no such tool existed for rapid, customizable protein degradation...both short-and long-lived proteins, and it can even distinguish between protein variants when used with ...addition of exogenous TRIM21 protein along with an antibody targeting the protein of interest led to rapid...Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing...Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...physiology, TRIM21 recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et...rapid, targeted degradation that is dependent on proteasome function. TRIM-away compared favorably to drug...

...'re using green fluorescent protein or Enhanced Green Fluorescent Protein (GFP/EGFP) for a transcriptional...However, if you fuse your protein of interest (POI) to GFP to study the protein's behavior in cells, in solution...interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while a protein with a high...with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize can...  1. Transmembrane fluorescent protein fusions Transmembrane proteins, i.e. receptors and transporters...lead to polymerization of the fusion protein. A dimeric fusion protein will have two FP binding domains forming...formation as fusion protein concentration increases. The polymerized fusion proteins that form as a result...

...powerful approach to control protein-protein interactions in cells because the protein binders can be customized... Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with a 2in1 vector...The 2in1 cloning system is well suited for protein-protein interaction (PPI) studies in plants that rely...25971551. Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed...photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change in...technique using small scaffold proteins to identify novel binding proteins that bind to either the light...applications of protein partners that bind to only the light- or dark- state of the photoswitchable proteins offers...

...segment The human proteome contains a breadth of modular domains that facilitate protein-protein interactions...compartments is via proteomics--the study of the distribution and interactions of proteins within these structures...significantly stronger phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds...distribution and interaction networks of specific proteins in living cells. Until recently, the enzymes most...APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires H2O2, which can be...TurboID and miniTurbo makes PL a viable method for proteomics in model organisms in which it was previously...expressed. In this case, the gene encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and...

...synaptic cleft proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand...particular proteins. Browse or search the table below to find antibodies targeting V5, Protein C, DYKDDDDK...secreted proteins from a collaboration between Addgene and IPI. Antibodies... Antibodies Institute for Protein Innovation...Innovation Antibody Collection Institute for Protein Innovation Antibody Collection Axon Guidance Antibodies... in a new window) Welcome to the Institute for Protein Innovation (IPI) Collection! Here, you’ll find ...IPI to generate antibodies to highly conserved proteins and other difficult-to-target antigens that are...

... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids ... Backbones Study your protein of interest by fusing it to a fluorescent protein. Michael Davidson Fluorescent...protein-protein interactions or conformational changes within a protein. Biosensors Monitor small biomolecules...and Explore Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...Addgene's repository contains thousands of fluorescent protein plasmids. Use this guide to learn more about the...

...is often used to study (1) protein-protein interactions where each protein is separately fused to a donor...to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...changes within a protein where the donor and acceptor are both fused to the same protein (also referred ...calibration standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity, affinity...and fast-maturing red fluorescent protein Variety of fusion proteins, positive controls for many FRET ...measure protein interactions or conformation changes. Plasmid... Plasmid Collections Fluorescent Proteins FRET Fluorescent...

...Rinehart Lab Phosphoprotein Reagents Rinehart Lab Phosphoprotein Reagents Phosphoprotein Synthesis Reagents...generate the new phosphorylated protein. Protein Phosphorylation Protein phosphorylation is one of the ...positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...phosphorylated proteins with one or more phosphorylation sites (Pirman et al., 2015). Improved phosphoprotein production...-P for screening phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 ...multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics . Nature Methods ...Robust production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications...

... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor for... Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator...pH-sensitive fluorescent protein pHmScarlet . pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis...of Redox-sensitive protein sensors (roGFP) Lifetime imaging of a fluorescent protein sensor reveals surprising...includes BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs) Yeast GPCR-sensor...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties ...

...Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian origin...different fluorescent tags for you to create fusion proteins with your gene of interest. Most of these plasmids...opens in a new window) for images, tutorials, protocols, and more. Empty Backbones Plasmid Description...photoconvertible, and photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase...fluorescent proteins. Plasmid...Fluorescence microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or...unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in live animals...background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome photoreceptors...vivo imaging tools in this blog post . Far-Red Protein Excitation (nm) Emission (nm) Brightness Plasmids...

...marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent protein like mCherry...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...pattern, you should fuse your protein of interest with a fluorescent protein that has a different emission...the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you...responsible for delivering proteins to the extracellular environment. Proteins are first translated at the...different fluorescent protein markers? Check out the Michael Davidson Fluorescent Protein Collection or search...Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last reviewed: 10...

...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along with key... Yeast Fluorescent Protein Collection Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct Kit...Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...create fusion proteins with your gene of interest. Plasmid... Plasmid Collections Fluorescent Proteins Empty Backbones...Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared...Tables are organized by color of the fluorescent protein. The content for this page was originally compiled...

...absence of a protein, determine if a protein is up or downregulated, examine a protein’s stability or ...Hjelm, 1972, Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose...support while unbound proteins are washed away.  While both Protein A and Protein G have an affinity for...compatible with either Protein A or G.  While commonly used, Protein A and Protein G are not suitable for...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the...of the target protein or interfere with protein interactions of interest. Tagged proteins are typically...modifications, or study how a target protein interacts with other proteins or nucleic acids. Read on to learn...

...step: protein degradation, incomplete lysing/insoluble proteins, and protein aggregation. Protein degradation...lysing protocol developed for isolating proteins from a specific compartment. If your proteins are nuclear...lysing protocol Subcellular fractional kit or lysing protocol Not enough target Run protein control...two types of gradients. Protein gradients use dilutions of your sample or protein of interest to understand...  Figure 1: A protein gradient. The density of the bands, which increase as protein concentration increases...which would result in low protein yield, can be reduced by adding a protease inhibitor cocktail and phosphatase...DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins from their...

...for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need ...for the amount of protein you are expecting.  Fluorescent proteins Fluorescent proteins, while more expensive...normalize your protein of interest to overall protein expression for each sample and protein. While ubiquitously...normalize your protein of interest to total protein expression. Total protein expression can be measured using...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments,...considered if your protein is difficult to solubilize, such as a membrane-bound protein. There are many ...