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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 799 results for: tec

Showing: 61 - 80 of 799 results

  1. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

  2. Antibodies

    Type
    Collection
    ...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...

  3. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity of ...

  4. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Viral Vector Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...inclusive, ‘open-access ethos’ to the results, techniques and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional protection added ...

  7. Microbiology Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

  8. Worm Expression Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

  9. AAV Packaged on Request

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. We offer 963 in-stock viral vector preps...well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality...

  10. Lentivirus Plasmids

    Type
    Collection
    ... used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253... used with 2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express ...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

  12. CRISPR Plasmids - Tagging

    Type
    Collection
    ...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Center at UCSF / QB3 Wiki Stepanenko et al. : BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327 ... No. 10, pp.1296-304 Kogure et al. : Nature Biotechnology, May 2006, Vol. 24 No. 5, pp.577-81 Shcherbo...

  15. TALEN Expression Vectors

    Type
    Collection
    ...wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat...

  17. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state...

  19. Video Library

    Type
    Protocol
    ...tips for mammalian cell culture Aseptic Technique Aseptic technique is a critical skill for success in numerous... the key strategies of aseptic technique, including personal protective equipment, setting up a clean ...Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once ...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals, cells,...
Showing: 61 - 80 of 799 results