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We narrowed to 145 results for: transfection

Showing: 61 - 80 of 145 results

  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...the passage number of a culture the lower the transfection and transduction efficiencies. Cultures should... while others opt for a single harvest after transfecting with the transfer and packaging plasmids. A ...

  2. Mammalian RNAi Tools

    Type
    Collection
    ...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...delivered as any type of small RNA and can occur via transfection or viral delivery of a plasmid. RNAi is an umbrella...mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro experiments...

  6. Adenovirus Plasmids

    Type
    Collection
    ...both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs...

  9. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...General Transfection Protocol Recombinant Antibody Purification Protocol Introduction Transfections allow...using Polyethylenimine Max as a transfection reagent. After transfection and expression, the recombinant...mL with >95% viability to proceed with transfection. Transfect the flask containing 108 mL cells: Transfer...feeding schedule: Thursday: Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (...post-transfection): Add valproic acid to 3.75 mM, add 4% BCD Feed. Tuesday (120 h post-transfection): Add...Wednesday (144 h post-transfection): Add 4% BCD Feed. Thursday (168 h post-transfection): Harvest antibody...protocol for transfection of HEK293 cells to produce recombinant antibody. Protocols...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...multimers may exhibit reduced transformation and transfection efficiency compared to the monomeric version... Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian...

  11. General Transfection

    Type
    Protocol
    ...general method for transfecting mammalian cells using linear polyethylenimine. Transfections allow for transient... viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with...Carefully transfer the transfection mix to your HEK293T cells. Add the transfection mix dropwise, being ...protocol to transfect mammalian cells with your plasmid of interest. Protocols... Protocols General Transfection General...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...adapted for different cell lines and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T...

  15. Lentivirus Production

    Type
    Protocol
    ...lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol. This ...packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace...modified for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used...hydroxide 0.22 μm polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging plasmid(s),...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to... 2 for ~20 h. Prepare a mixture of the three transfection plasmids into OptiPro SFM: Reagent Amount per...transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid DNA purification should ...

  20. Brain Initiative Collection

    Type
    Collection
    ...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...
Showing: 61 - 80 of 145 results