We narrowed to 1,041 results for: ins
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TypeBlog Post... Aliyah Weinstein...
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Sensing Neuronal Dopamine
TypeBlog Post...surrounded by a semipermeable membrane, which is inserted into the region of the brain where DA quantitation... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit mission... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...research interests. “So for me, I researched G-proteins at the time. This program takes what you know ... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...pipette, it might make sense to earn a master’s instead of a PhD. Deciding to pursue a master’s is a big... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...determine the optimal localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, ... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...brick-by-brick”. Our mission is to help make science mainstream and to show people the very human struggles in... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...Said Blue Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post... Eric J. Perkins... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...Availability of superiors for consultation, advice and brainstorming. Employees must understand the part they play... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...resources where possible. At the lunch table, for instance, we have a plastic container problem…reusable ... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you... -
AAV Viral Preps
TypeCollection...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools ... -
CRISPR Plasmids - Parasites
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have... -
CRISPR Plasmids - C. elegans
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...start her own research group at Boyce Tompson Institute. References Baldwin, Melinda. "Credibility, peer... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in... -
Viral Vectors 101: Transductions
TypeBlog Post...so why and when should you opt to make a virus instead of just zapping your cells? That really depends...