We narrowed to 1,049 results for: ins

...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps...encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins, which...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...expression and localization. Many other types of proteins can also be controlled by light, generally by ...

...mCherry tagged fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector with ...designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning...and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization...backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more. Plasmid...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...function section for if you want to stably express an insert using viral vectors, are studying a different genetic...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...

...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that have homology... potential target sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids...2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given ...NGG PAM sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized...2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized...SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized...

...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties ...transfer (FRET) efficiency of a pair of fluorescent proteins. Genetically encoded biosensors can be introduced...Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul...nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...

... monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount...of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...to highly conserved proteins and other difficult-to-target antigens in the Institute for Protein Innovation...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights ...1alpha-driven constitutive transgene expression. Contains intron that gives high expression. Didier Trono...and Paul Kaufman 102930 pLJC5-Tmem192-3xHA 3rd Contains a lysosomal tag. See Abu-Remaileh et al. (2017...expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch...and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , vif , and nef . Can be...transfer plasmids. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains gag and pol . Can be used with 3rd generation ...

... Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter... Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/Insert Promoter...table for "Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Purify ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Visualize ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

...small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote...including point mutations, base changes, and large insertions or deletions. For more information on the wide...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their activity...sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations using a prime... In many cases, you can synthesize, anneal, and insert the targeting oligos into plasmids containing the...can generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile...

...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Publication Return to top Parasite ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

...Plasmid Gene/Insert Vector Type PI Return to top Bacterial Strains Browse bacterial strains that have been...synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic code in bacteria... Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for all life is based upon four...properties for studying, manipulating, and evolving proteins. Expanding the Genetic Code During protein translation...genetic code can help us understand and evolve proteins for various purposes from therapeutics to biopolymers...phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic code expansion without buildup of...DE3) ΔserC serC gene knocked out; for expressing proteins containing site-specific non-hydrolyzable phosphoserine...

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity... constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting ...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...

...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 ...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...#68307) ) to generate the phosphosites with serine instead of phosphoserine (i.e., a “phosphorylatable” phosphosite...

...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

...Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent...Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...more about the many applications of fluorescent proteins and to find plasmids for your next experiment....collection of genes and markers tagged with fluorescent proteins as well as hundreds of empty backbones. FRET Find...subcellular structures with well-characterized marker proteins. In Vivo Imaging Image with far-red or infrared...open-source, community-editable database for fluorescent proteins created by Talley Lambert. Nikon MicroscopyU (...

...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...is important. However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end...