We narrowed to 1,049 results for: ins

...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection....Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...associations. Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit Plasmids containing modular inserts that span...reaction Bunz Return to top Adenoviral Strains Browse bacterial strains for adenoviral vector production. ...plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production in Addgene's repository...Shuttle Plasmids Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery... Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...pUMVC Contains MoMLV gag and pol . Use with an envelope plasmid. Bob Weinberg 12371 pCL-Eco Contains MoMLV...includes a reverse transcriptase and an integrase to insert the viral genome into a host cell. Scientists can...packaging genes Envelope plasmid — contains packaging gene env This collection highlights popular gamma-...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672 MSCV-IRES-GFP MSCV...pol , and env . Inder Verma 35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top...

...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...responsible for delivering proteins to the extracellular environment. Proteins are first translated at the...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...assembled a collection of popular fluorescent fusion proteins that are targeted to various organelles or subcellular... (find plasmids for cloning in our Fluorescent Proteins Empty Backbones Collection ). Then, by examining...p63 mTurquoise2 Dorus Gadella 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...F-tractin-EGFP Actin filaments F-tractin EGFP Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell...

...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase...fluorescent proteins. Plasmid...Fluorescence microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or...unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in live animals...background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome photoreceptors...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging...Chemogenetics Fluorescent Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide...

...Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden...environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain...terminators, repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab...Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning ...community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering Research...

...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal D omain...or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...Additional Resources (Link opens in a new window) BrainSpan Atlas of the Developing Human Brain - includes... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA...sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve...Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506 pOpen-Eco31I Eco31I... Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource..., and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's disease research. This collection is part of ...search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...to amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked...

...Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI ...Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI... table for "Bacteria". Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base Edit...Publication Return to top Purify ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria...genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin...

...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled ...credit: Maya Kostman for the Innovative Genomics Institute The Coronavirus Disease-2019 (COVID-19) pandemic... plasmids expressing genes encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E;...Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...

...fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are...collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive...promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources...2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https:/...

...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along with key...create fusion proteins with your gene of interest. Plasmid... Plasmid Collections Fluorescent Proteins Empty Backbones...Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared...are prone to dimerization at high concentration. Proteins that are derived from GFP and contain the A206K...Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish/Xenopus/Worm...Expression Return to top Long Stokes Shift These proteins have a large gap between their excitation and ...

...useful for detecting and tracking key drug target proteins....operational complexity. This unique collection contains plasmids developed by Promega scientists and designed...biology experimental workflows. The collection contains luciferase reporter vectors for sensitive gene...protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, SmBiT, and...SmBiT and expressed in live cells. When the two proteins interact, the subunits come together to form an...exceptional dynamic range can detect low-abundance proteins at endogenous levels in lysates or live-cells,... and inhibition in real time using full-length proteins or fragments. NanoBRET target engagement (TE) ...

...recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences into the open reading...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...intersectional experimental design. The Deisseroth Lab maintains a Standard Operating Procedure (Link opens in ...transgenic mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...

...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools ...somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time... lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic loci in... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...Center (ZIRC) - A US-based repository for zebrafish strains and materials and information about zebrafish research...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...