We narrowed to 1,041 results for: ins
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TypeBlog Post...Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation...
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Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...network Attend informational interviews to gain insight into the daily routine of the potential job Join... -
Plasmids for Stem Cell Research
TypeCollection... L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more efficient method...regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used...name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions... -
TALEN Guide
TypeCollection...for targeting enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists...consisting of 12-31 repeats can be assembled and inserted into a variety of backbones in just a few steps...Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available from Addgene before the end...Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas DF. Science... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...can also request the specific serotypes you need instead of receiving a kit with all the serotypes, since... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization... -
Addgene Depositors Get More Citations
TypeBlog Post...journals as those that did make deposits to Addgene. Insomuch as different journals represent different research... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...topics and interests. Offer help when you can, instead of just asking for favors. Don’t focus on networking... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post... consensus to the next meeting of experts, and instead convene the necessary groups for the necessary ... -
Science Career Options
TypeBlog Post...available and then decide on a course of action. Instead of rushing in, you take steps to make it more likely... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019.... -
Management for Scientists: Delegating is Key
TypeBlog Post... the end product would likely be poor quality. Instead, we pull together teams of individuals who enjoy... -
Plasmids 101: Inducible Promoters
TypeBlog Post...regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...AAVrg while other pathways such as those in the brainstem were not. Others have noted a lack of transduction... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post... using your single color controls (Figure 1A), instead of using your actual samples. They may also be ... -
How Addgene Creates Content
TypeBlog Post...careers Plasmids CRISPR Viral vectors Fluorescent proteins ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...which often focuses more attention on the results instead of the methods. Unreported flexibility and questionable...