We narrowed to 1,015 results for: LEA

1. Tips for Using BLAST to Verify Plasmids

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   ...: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources...

2. Antibodies 101: Producing Recombinant Antibodies

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   ...quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies...

3. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https...

4. Getting to Know Expansion Microscopy

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   ...This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible...

5. SciComm: Taking Science to Elementary and Middle Schools

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   ... their minds, but I think many of the students learned something. Based on the initial guidelines I received...

6. Simplify Cloning with in vivo Assembly

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   ...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...

7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter...

8. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...

9. Plasmids 101: Shuttle Vectors

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   ...the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector...

10. CRISPR 101: Any Base Transversion Editors

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    ...Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil...

11. Plasmids 101: Stringent Regulation of Replication

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    ... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

12. Plasmids 101: Broad Host Range Plasmids

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    ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

13. Viral Vectors 101: Preparing Pooled Libraries

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    .... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ...

14. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know...

15. To Codon Optimize or Not: That is the Question

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    ... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural...

16. Chemogenetics Guide

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    ...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...For a discussion of alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD...(Fz) or chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics...

17. An Integrin Antibody Toolkit from IPI

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    ...integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While...

18. Five Popular Model Organisms

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    ...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J...

20. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...PMC4737176. Additional Resources on the Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits...