...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...

...replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter...

...the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...

...Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil...

... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

...first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know...

... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural...

.... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ...

...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...genome editing. For more information on AAV-CRISPR, please see this blog post. Browse the article from Ran...particles produced during a lytic cycle are quickly released through cell lysis. Episomal stability enables...

...integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While...

...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...

...Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...For a discussion of alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD...(Fz) or chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics...

...PMC4737176. Additional Resources on the Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits...

...editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless: genetic...

... by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed...

...structural understanding of your protein that can lead to novel insights about its biology and its behavior...

...development. eBooks Download any of our eBooks to learn about popular topics related to our collections....Plasmid Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction...

...created the following guides to help you start learning about various scientific concepts related to our...Read More Viral Vectors Find educational guides to learn more about the different types of viruses that are...