We narrowed to 1,026 results for: LEA

1. Of Myc and Men

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   Blog Post

   ...science happens in a vacuum!  Munro and Pelham learned of these new antibodies and identified Myc1-9E10...

2. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   ... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

3. Tips for Using BLAST to Verify Plasmids

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   ...: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources...

4. Antibody Guide

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   Guide

   ...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

5. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies...

6. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... their minds, but I think many of the students learned something. Based on the initial guidelines I received...

7. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https...

8. Getting to Know Expansion Microscopy

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   ...This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible...

9. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter...

11. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...

12. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post

    ... based on the wear-and-tear footprint that they leave on the DNA only in target cells. This pattern is...

13. Plasmids 101: Shuttle Vectors

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    ...the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector...

14. CRISPR 101: Any Base Transversion Editors

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    Blog Post

    ...Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil...

15. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

16. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know...

17. To Codon Optimize or Not: That is the Question

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    Blog Post

    ... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural...

18. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    .... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ...

19. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

20. Adeno-associated virus (AAV) Guide

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    Guide

    ...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...recombination between the transfected plasmids could lead to the production of replication-competent viruses...element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog... packaged in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics...stimulates the expression of transgenes via increased nuclear export. Poly(A) signal in cis The polyadenylation...polyadenylation or poly(A) signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random insertion...