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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 987 results for: des

Showing: 961 - 980 of 987 results

  1. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules. The...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which...next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...precipitation, and resuspension. This protocol provides two options: using Solution D (see reagent section...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  5. Addgene's eBook Collection

    Type
    Guide
    ...First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About... tips for planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide...November 2018 | First Edition About The guide includes information on the many career options in science...

  8. Fluorescence Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...

  10. Colony Formation Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S...PMID: 24501411 Gilroy, K. L., Terry, A., Naseer, A., De Ridder, J., Allahyar, A., Wang, W., Carpenter, E.... Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral...been widely used in the research community for decades. Wildtype gamma-retroviruses have been engineered...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer) are ...into the HEK293T packaging cell line. This system provides the greatest flexibility to pseudotype gamma-retrovirus...

  15. CRISPR Guide

    Type
    Guide
    ...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...Han, Y., Ogrodowski, P., Crippa, A., Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014)...., Boix, C., Perrouault, L., Tesson, L., Geny, S., De Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli... the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit..., the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT template. The...

  16. Plan Your Experiment

    Type
    Guide
    ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...protocol. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases,...experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment...

  17. Molecular Biology Reference

    Type
    Guide
    ...helix. The order of these 4 nucleotides makes up the genetic code and provides the instructions to make ...thymine in RNA molecules. Every 3 nucleotides (codons) in a DNA sequence encodes for an amino acid. The genetic... transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine, thymine, cytosine, and guanine...amino acids. Each amino acid is encoded for by 3 nucleotides termed a codon. As there are only 20 natural ...essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance...some common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...

  18. Chemogenetics Guide

    Type
    Guide
    ...a new window) Guettier JM, Gautam D, Scarselli M, de Azua IR, Li JH, et al. (2009). A chemical-genetic...less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...Wess J (2012). Design and functional characterization of a novel, arrestin-biased designer G protein-coupled... Science Guides Chemogenetics Guide Chemogenetics Guide Jump To... alternative DREADD ligands. Compound 21, Deschloroclozapine (DCZ), Perlapine, and Olanzapine have all...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD...

  19. Antibody Guide

    Type
    Guide
    ... is described in more detail in the Multiplex Assays section below. The following table describes the ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...

  20. Lentiviral Vector Guide

    Type
    Guide
    ... Interchangeable; usually encodes for VSV-G Interchangeable; usually encodes for VSV-G Safety Safe; replication...integration of the barcodes, which can then be inherited by daughter cells. Inheritance of the barcodes makes lentiviral... Science Guides Viral Vectors Lentiviral Vector Guide Lentiviral...been widely used in the research community for decades. Wild-type lentiviruses have been engineered into...ONLY by a second-generation packaging system that includes the tat gene Can be packaged by both second- and...and eliminates the requirement for Tat. Always includes deletion in the 3' LTR for self-inactivation LTR...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...
Showing: 961 - 980 of 987 results