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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 404 results for: pho;

Showing: 41 - 50 of 404 results

  1. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express...

  2. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...non-radiatively transferred from an excited donor fluorophore to an acceptor. Since the transfer of energy...energy does not occur by emission of a photon, the acceptor molecule is not required to be fluorescent. A...

  3. Control AAV Preps

    Type
    Collection
    ...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...

  4. Viral Production

    Type
    Collection
    ...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...

  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...configuration have been developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded...H134R)-eYFP Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ... Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec ...Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells. Cell Stem Cell. 2015 Nov 5;17...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...wavelengths of emitted photons are higher than those of absorbed photons. When photons excite electrons of... you'll need to pick a fluorophore for each target and ensure the fluorophores, together, are able to ... about 380–700 nm. Photons of lower wavelengths are higher in energy, while photons of higher wavelengths...whereby a photon is emitted. This step is called emission. During excitation, some of the photon's energy...the energy of an emitted photon is lower than the energy of the absorbed photon. Correspondingly, wavelengths...outlining a few commonly used fluorophores and their emission spectra. Some fluorophores carry their emission ...and RB545. Fluorophores can also come in combination of two conjugated single fluorophores, like APC-Cy7...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...design utilized Magnet photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa...split fusions and generate a photoactivatable nickase and a photoactivatable repressor (dCas9). The activity...Off Genome Editing Single-chain Cas9 photoswitches Photoactivation of Cas9 can also be achieved using single-chain...chemistry to photocage CRISPRs The aforementioned techniques each employed a photoactive strategy which...Comparison of photoactivatable CRISPR strategies Publication System nickname Photoactivatable moiety Cas9...et al., 2016 CRISPR-plus Photocleavable ssDNA oligonucleotides Cas9, photocaged-gRNA Gene editing Manna...optogenetic techniques to develop a variety of photoactivatable CRISPR tools. These tools allow scientists...

  9. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...proteins predicted to be phosphorylated on at least one site. Phosphorylation is particularly important...predict. Slower Dephosphorylation Rates: FRET-based reporters can have slower dephosphorylation rates than ...by FRET reporters having phosphorylated conformations that are less phosphatase-accessible. Kinase Translocation... signal (NES), both which are phosphorylatable. Prior to phosphorylation, the KTR localizes to the nucleus...biosensors Learn about using phosphoserine to study protein phosphorylation Additional Resources on Addgene.org...specific spectral properties that limit which fluorophores can be multiplexed together. Challenging  to...nucleus, but following phosphorylation bNLS activity is suppressed and NES activity is enhanced, leading...

  10. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...fluorescent proteins or dyes. 3. Two-photon microscopy Two-photon excitation microscopy is a fluorescence...decypher cellular dynamics in lymphoid organs. In lymph nodes, for example, two-photon microscopy allowed scientists...worthwhile to test the photostability of your fluorescent protein when using 2-photon microscopy as this ... is advantageous when compared to photoactivable or photoconvertible proteins, as it enables tracking ...mobility with movement from the bone marrow and other lymphoid organs to specific tissues where they can scan... good tools to directly follow immune cells in lymphoid organs. The development of cell sorters together...several labs were able to decipher the journey of lymphocytes from the bone marrow to the thymus where they...
Showing: 41 - 50 of 404 results