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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 968 results for: LAT;

Showing: 81 - 100 of 968 results

  1. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ...insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator peptide ...Purify dCas9 fused to an epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence...

  3. Antibody Production

    Type
    Collection
    ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...binding site (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 — introduction of five...

  5. Plant Plasmids and Resources

    Type
    Collection
    ...technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...

  7. CRISPR References and Information

    Type
    Collection
    ...and correspond to edited and non-edited cell populations. For more information, see the Hsiau et al. (.... protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR knock-in design at scale. It uses an...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ... the first plate to get a good idea of what that volume looks like in your particular plate. For the remainder...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

  9. Brain Armamentarium

    Type
    Collection
    ...AAV-mediated transgene expression in specific populations of brain cells The Allen Institute for Brain ...SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons Jonathan Ting Viviana...SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana...

  10. Protocol - How to Streak a Plate

    Type
    Protocol
    ... and Isolating Bacteria on an LB Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation... last section of the plate, to create streak #3. Incubate plate with newly plated bacteria overnight (... DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria from...single colony from a freshly streaked agar plate to inoculate a bacterial culture for DNA purification ...explains how to isolate a single bacterial colony by streaking it onto an LB agar plate. Last Update: Feb... LB agar plate (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate... one edge of the plate. Then streak horizontal lines in another section of the plate, and then diagonal...

  11. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... Protocols Inoculating a Liquid Bacterial Culture Inoculating a Liquid... may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks...culture bacteria in the lab. LB agar plates are frequently used to isolate individual (clonal) colonies of ...necessary to isolate enough plasmid DNA for experimental use. The following protocol is for inoculating an overnight.... You can now isolate your plasmid DNA from the bacterial culture by following Isolating Your Plasmid ...your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing... for Inoculating a Bacterial Culture Protocols...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation...device that fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn of E. coli...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...purpose, provided that they fit your petri plate. Incubate the plate at room temperature for at least 30 minutes...selection. 150 µL of 0.1 mg/mL Carbenicillin plated over-agar Plate shows a lawn of E. coli and no apparent...selection. 150 µL of 1 mg/mL Carbenicillin plated over-agar Plate shows several individual colonies and effective...

  13. Synthetic Biology - Strains

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search the table...

  14. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing...

  15. Synthetic Biology - Metabolism

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include...

  16. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library construction...

  17. KLF Research Plasmids

    Type
    Collection
    ...Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi Michael Ruppert...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

  18. Depositor Collections

    Type
    Collection
    ...Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs Community (MCC) Collection ...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt...

  19. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...CRISPR plasmids for activating or upregulating gene expression in various model organisms.... to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid...

  20. MAPK Plasmids

    Type
    Collection
    ... signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...
Showing: 81 - 100 of 968 results