Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 111 results for: lentiviral dna vectors

Showing: 81 - 100 of 111 results

  1. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated into...γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results in long-term...ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...called DNA transposons. DNA transposons are commonly used as tools in the lab, so we’ll focus on DNA transposons...Transposon: The transposon is the DNA sequence that moves around. This DNA sequence encodes the proteins ...BioRender.com.   Class II TEs: DNA transposons Class II TEs are also known as DNA transposons, since they do...break, and excises the transposon from the donor DNA. A DNA footprint is left behind. When the transposon-transposase...not leave behind a DNA footprint sequence after excision. piggyBac can mobilize DNA over 100 kB in size...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...TnsB, TnsC), and 4) the donor DNA or mini-transposon that contains the DNA cargo of interest flanked by...

  3. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178...the edited strand and wild-type strand of the host DNA. When these strands hybridize, the nuclease can detect...account other elements, including those relating to DNA structure (e.g. epigenetic modification, bulges) ... et al. "High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity...Frock, Richard L., et al. "Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases... Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  4. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ...or AAV. Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The..., a viral vector is selected to perform the transduction with: either retrovirus, lentivirus, adenovirus...bacterial in nature. All three of these methods involve DNA incorporation into the cell, but the mechanisms vary...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...layers of risk management when working with viral vectors. Typically, one day after transfection into the...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...delivered in other forms (mRNA, DNA, etc.) to raise immunity. Viral vectors are being explored as the delivery...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical...want delivered to your target cells. This delivered DNA/RNA can then be either permanently integrated into... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...nucleic acid cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies... improved specificity. AAV vectors are able to facilitate and enhance DNA repair via homology directed...makes AAV vectors especially amenable to correcting disease-associated mutations. AAV vectors maintain... Check out our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV...thereby correct the mutations using retroviral vectors. However, many diseases require techniques that...usability in clinical settings. On the other hand, vectors derived from AAV have recently gained popularity...profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are a variety ...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+...mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP...93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow Protein...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy...-associated viral vectors: packaging capacity and the role of rep proteins in vector purity." Human gene...Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research... AAV vectors without specific facilities (Biosafety Level-1). Other possible tools, lentiviruses for example...Immune Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount...Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting my graduate program at the...in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic/optogenetic constructs ...

  9. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing... contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...they physically deposit their cargo into the host’s DNA. These viruses also have a propensity to integrate...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...on the functional elements they contain. Plasmid DNA in both prokaryotic and eukaryotic systems must be...the right. The tertiary structure of the hairpin-DNA complex is thought to destabilize the transcription...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: 10074136...Transcription Termination Rho-dependent termination DNA transcription Polyadenylation: General overview... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

  11. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...receptor could be something like an antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources ...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...., Human induced pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928...mouse induced pluripotent stem cells without viral vectors. Science, 2008. 322(5903): p. 949-53. PubMed PMID...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...

  14. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...images of brain regions, or sequences of microbial DNA they all seem to require some mathematical know-how...to generate new knockout mice, using adenoviral vectors to deliver IGF receptors to the brain, and probing...individual labs to make efficiently. For select viral vectors in the repository, you can instead have Addgene... into neuroscience, you could look to our viral vector pages on the Addgene website and on the Addgene...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...to be digested with DNase before undergoing titering, to remove residual plasmid DNA carried over from ...you’ll need to determine the titer of your viral vector solution. A variety of titering methods have been...about common AAV applications by reading our Viral Vectors 101 series!  This post was originally written by... Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/...the Stability and Inactivation of AAV Serotype 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. ...(2021). Inactivation of Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy...single-stranded and self-complementary adeno-associated viral vector genome titers by droplet digital PCR. Human Gene...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ... how proteins interact with one another (or with DNA) to perform diverse biochemical tasks in the cell...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of...-IN” bioinformatics tool for guide RNA and donor DNA design, and implemented our optimised protocols into...to cells: a guide RNA, Cas9 protein, and a donor DNA that contains the tag sequence flanked by homology...CRISPR experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also...csRNP complex formation, a single-stranded donor DNA (ssODN) that contains the tag-encoding sequence and... annealed cr:tracrRNA, and single-stranded donor DNA. The success of the epitope tagging experiment depends...

  17. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ... Separate DNA by size on an agarose gel Watch the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial... the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification...Measure DNA concentration with a spectrophotometer Restriction Digests Cut and analyze plasmid DNA with ...FAQ Watch the Video! Purifying DNA from an Agarose gel Isolate specific DNA bands cut from a gel Watch the...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ... Introduce plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May...motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...

  19. CRISPR References and Information

    Type
    Collection
    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...

  20. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395...Inhibition Mouse Weissman 3rd 5 10 107,415 214,830 DNA Damage Response MKOv4 Library 140219 Knockout Human...110066 Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...
Showing: 81 - 100 of 111 results