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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 389 results for: ARIA

Showing: 201 - 250 of 389 results

  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...

  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...

  7. Resolute Plasmid Collection

    Type
    Collection
    ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded... the nucleus, and several codon optimized Cas9 variants have been created in an effort to increase translation...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...

  15. Antibody Production

    Type
    Collection
    ...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

  16. Antibody Plasmid Collection

    Type
    Collection
    ... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...

  17. mTOR Pathway

    Type
    Collection
    ...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now the most popular...any cloning at all! As mentioned above, several variants of these tags have been developed over the years...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...

  22. Plant Plasmids and Resources

    Type
    Collection
    ...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...

  23. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...

  24. TALEN Guide

    Type
    Collection
    ...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...

  25. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... loop to produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo...Components of CRISPR  xCas9: Engineering a CRISPR Variant with PAM Flexibility Cas9 vs the Other Cases Further...

  26. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

  27. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...et al. A percentage is not listed because many variables (cell type, criteria used, etc.) can effect reprogramming...insertional mutagenesis and random integration creates variation between iPSC clones. Retroviruses are not considered...

  28. TALEN Plasmids and Kits

    Type
    Collection
    ...GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed...

  29. COVID-19 Resources

    Type
    Collection
    ...Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein RBD mutations for comprehensive mapping...

  31. Luciferase Plasmid Collection

    Type
    Collection
    ...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate...

  33. Plan Your Experiment

    Type
    Collection
    ... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...

  36. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  42. What's New in CRISPR - May 2019

    Type
    Blog Post
    ... in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases...
Showing: 201 - 250 of 389 results