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Addgene
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  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...protein of interest, there is also a protease recognition site. In unperturbed conditions, the protease...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...proper protein expression elements (ribosome recognition sequences, start codon, stop codons, reading ...

  21. Lab to Office Culture Shock

    Type
    Blog Post
    ...lentivirus production, I didn’t really start to recognize my transferable (or “soft”) skills until I was...

  22. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...original V5 tag mouse monoclonal antibody, which recognizes a short, linear epitope derived from the P and...

  24. Bioinformatics at Addgene

    Type
    Blog Post
    ...the steps into a continuous pipeline. We also recognized the software system that we chose needed to fulfill...

  26. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...may impose some limitations and you should be cognizant of these when interpreting your results. The key...

  31. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...the 13 amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence...

  33. Evolution of Lab Techniques

    Type
    Blog Post
    ...anemia. The mutation happens to destroy a sequence recognized by the restriction enzyme MstII. When a restriction...

  41. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...epitopes such that immune complexes will no longer recognize them. Sometimes, users will combine methods and...
Showing: 201 - 250 of 258 results