Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 89 results for: 5-Oct

Showing: 81 - 89 of 89 results

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...administered at a total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector...across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912–921. https://doi.org/10.1016/j.ymthe.2019.02.013...targeting distinct brain cell types. Nature Methods, 17(5), 541–550. https://doi.org/10.1038/s41592-020-0799.... R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer, J., Winchester...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...a lab is nothing like a typical part-time or 9-to-5 job. I had to ask to find out what a “postdoc” is,...teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific...scientific and professional training after earning a doctorate Considered a "stepping stone" position to become...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...PMID: 25998715. PubMed Central PMCID: PMC4491443. 5. Scott, Andrew M., Jedd D. Wolchok, and Lloyd J. Old...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at Binghamton University. She works ...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...resuspended cell pellets and sonicate 5 x 1 sec pulses with at least 5 minutes on ice between each pulse,.... T-175 flask, Corning 430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269...high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine... water + 100 mL of 1 M Tris HCl pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride...supernatant and resuspend the pellets in a total of 5 mL of cell lysis buffer (recipe above). Pipet back...before these images were taken. Last reviewed on: October 10, 2023...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate...at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from...micrograms of antigen loaded. Last reviewed on: October 8, 2024...

  9. Optogenetics Guide

    Type
    Guide
    ...processes via protein engineering. Biochem Soc Trans. 41(5): 1183–1188. PMID 24059506 Klapoetke NC, Murata Y,...inhibition of neuronal activity in vivo. Sci Rep. 5:14807. PMID 26443033 Wietek J, Prigge M. 2016. Enhancing...Red-light drivable channel from Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2...
Showing: 81 - 89 of 89 results