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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 724 results for: ACE

Showing: 81 - 100 of 724 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...OFP (Antares), and Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1...Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 ... in secreting proteins from the cell to the extracellular environment. Proteins are first translated at...

  3. p53 Pathway

    Type
    Collection
    ...is activated through both phosphorylation and acetylation, which disrupt its interaction with negative ... E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G Growth...also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1 p14ARF ...

  4. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...interest. Available modifications include histone acetylation by p300, histone demethylation by LSD1, cytosine...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... Light-Inducible Gene Editing and Transcription. ACS Chem Biol 13:443–448 . https://doi.org/10.1021/acschembio...With their light-activated CRISPR/Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy...optimal fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN...Far-red light, however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST ...Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana dCas9 Activation ...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...secretion, and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB...

  7. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...targeted epigenetic modification, including histone acetylation/demethylation, and cytosine methylation/demethylation...interest. Available modifications include: histone acetylation by p300 histone demethylation by LSD1 cytosine...

  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier.... Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug...

  9. Institute for Protein Innovation

    Type
    Collection
    ...recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration between...recombinant antibodies targeting families of cell surface and secreted proteins (glypicans and integrins)...

  10. Resolute Plasmid Collection

    Type
    Collection
    ...research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to the global benefit...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...

  11. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped virus particles can then be used to... to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round...

  12. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...specific vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...OZ563 and OZ564 CODA CODA tAGCTACCGCTGGCGTCTGAGGAGc HACE1 OZ565 and OZ566 CODA CODA gCTCATCTCCACCGAGCTGGAGAAg...

  13. Microbiology Resources

    Type
    Collection
    ... sp. Neisseria sp. Pseudomonas sp. Ralstonia solanacearum Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus...Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc) gene knockout and complementation...

  14. AAV Packaged on Request

    Type
    Collection
    ... for the banner on an eligible plasmid page and place your request. We will send you an email within 1...After approval, the next step is yours: you must place your order to begin production. You can do this ...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

  16. Viral Production

    Type
    Collection
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ...Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini et al. iScience. 2023 Apr 11...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....

  18. Rett Syndrome

    Type
    Collection
    ... RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...vector contains an insert (MAT2A) which can be replaced by another gene of interest. tTA-Advanced Tight...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the newest versions...Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding...that is quick, versatile, and robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector,...the CRISPR system. The ease and flexibility of the LACE technology makes this system widely accessible for...thereby creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...
Showing: 81 - 100 of 724 results