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We narrowed to 184 results for: CO

Showing: 81 - 100 of 184 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm ... in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm....

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled...

  7. Lentivirus Plasmids

    Type
    Collection
    ... EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... packaged in separate rAAV vectors which are then co-delivered to the host cells. The full-length transgene... sequence at its 5' end. When these viral vectors co-infect a cell, they form concatemers and splice together...both sequences present substantial sequence overlap. Co-expression induces homologous recombination and expression...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...2xNLS) fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort... in response to blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that...well as other photoactivatable proteins like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides...purify your POI, to perform pull down experiments like co-IPs and ChIP, and more - all without the need to ...specific genomic locus. When fused to the sgRNA and co-expressed with dCas9 – the dCas9, sgRNA/RNA cargo...

  17. CRISPR Guide

    Type
    Guide
    ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

  19. Hot Plasmids - May 2022

    Type
    Blog Post
    ...protein of interest. This fusion protein can then be co-expressed in essentially any transgenic zebrafish...
Showing: 81 - 100 of 184 results