...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and tetracycline... Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru ...transactivators that can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System...transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression...

...separate plasmid that does so; however, these independent gRNA plasmids can be paired with a wide variety...empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can search ...Marraffini pgRNA-bacteria 44251 Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...Qi pRPR1(1xTetO)_gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI...pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156...60901 Mammalian U6 yes, FokI S. pyogenes Conklin pcrRNA.ind 61284 Bacteria araB none S. pyogenes Beisel pcrRNA.con...

...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...because the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...additional 'G'. Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer... colonies, then genotype them, it is possible to find both single-allelic and bi-allelic cells. Single-allelic...double nickase system, with PX335 vector, you need to find two guides for each cleavage site, but this system... we usually pick 3-6 guides around the region to find the most efficient guide (most guides would work...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

... each library can be found on the individual library page. Find Pooled Libraries of Interest Click...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout... Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout...234820 Knockout Mouse Ebert 3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

...promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA...decreases as RNA is degraded within the cell Short window of CRISPR component expression may decrease off-target...Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target effects...PAM sequence is absolutely necessary for Cas9 to bind target DNA. As such, one can start by identifying...DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation...

... the applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing...applications of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein...Imaging Image with these powerful tools to study individual plasmids or protein-protein interactions in organs...

...vivo imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids...-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670...Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation)...

...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a...

...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...

...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...

... ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA binding domain,...the receptor to specific DNA sequences. Ligand binding triggers a conformational change in the receptor... an Addgene scientist. Nuclear Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene...

...scientists. They are generated using standard methods individually optimized for each specific antibody in order...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage.... cited in the Tissue Atlas (Link opens in a new window) . Western Blotting (WB) Proteins are extracted... or the Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does...

...Medicine Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...