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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,079 results for: IND

Showing: 81 - 100 of 1079 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit... is truncated at both the N and C terminus and induces mutation at rates much higher than the parental...expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with strong nuclear localisation...TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...architecture has been shown to increase mutation induction when using TALENs. The FokI domains (DD, RR) used... protein (previously shown to preserve the DNA-binding ability of TALEs), 63 wild-type TAL amino acids...

  2. Plasmid Collections

    Type
    Collection
    ... part of a pathway or ORF. Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics...and depositor collections on one page to help you find what you're looking for. If you are looking for ...based on type or application. Browse our guides to find the plasmids and science information you need before...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established a common...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Systems Biology Lab website (Link opens in a new window) References Botman D, de Groot DH, Schmidt P, Goedhart...019-38913-z. PubMed 30783202 (Link opens in a new window) Lee S, Lim WA, Thorn KS. Improved blue, green,...journal.pone.0067902. PubMed 23844123 (Link opens in a new window) Loqué D, Lalonde S, Looger LL, von Wirén N, Frommer...nature05579. PubMed 17293878 (Link opens in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent.../yea.1130. PubMed 15197731 (Link opens in a new window) Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Dual...2859-12-96. PubMed 24161108 (Link opens in a new window)...

  4. AAV Molecular Tools

    Type
    Collection
    ...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and tetracycline... Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru ...transactivators that can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System...transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...separate plasmid that does so; however, these independent gRNA plasmids can be paired with a wide variety...empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can search ...Marraffini pgRNA-bacteria 44251 Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...Qi pRPR1(1xTetO)_gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI...pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156...60901 Mammalian U6 yes, FokI S. pyogenes Conklin pcrRNA.ind 61284 Bacteria araB none S. pyogenes Beisel pcrRNA.con...

  6. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information...

  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

  9. Brain Armamentarium

    Type
    Collection
    ...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

  10. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... each library can be found on the individual library page. Find Pooled Libraries of Interest Click...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout... Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout...234820 Knockout Mouse Ebert 3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

  14. Fluorescent Protein Guide

    Type
    Collection
    ... the applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing...applications of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein...Imaging Image with these powerful tools to study individual plasmids or protein-protein interactions in organs...

  15. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...vivo imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids...-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670...Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation)...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a...

  17. Resolute Plasmid Collection

    Type
    Collection
    ...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...

  18. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...

  19. Antibody Production

    Type
    Collection
    ...scientists. They are generated using standard methods individually optimized for each specific antibody in order...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage.... cited in the Tissue Atlas (Link opens in a new window) . Western Blotting (WB) Proteins are extracted... or the Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Medicine Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...
Showing: 81 - 100 of 1079 results