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We narrowed to 1,083 results for: IND

Showing: 81 - 100 of 1083 results

  1. Antibody Plasmid Collection

    Type
    Collection
    ... optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...are large proteins made by the immune system that bind to an antigen, a particular part of a foreign substance...useful to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use...cells. Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example ... often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system... or ScFvs. Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...

  3. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...COVID-19 Plasmid Collection (Link opens in a new window) Researchers developing novel diagnostics, therapeutics...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...early 2020, Ginkgo Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities...plasmids available to scientists in academic and industrial organizations. For more information on ordering...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or.... Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based ...(Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit... is truncated at both the N and C terminus and induces mutation at rates much higher than the parental...expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with strong nuclear localisation...TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...architecture has been shown to increase mutation induction when using TALENs. The FokI domains (DD, RR) used... protein (previously shown to preserve the DNA-binding ability of TALEs), 63 wild-type TAL amino acids...

  5. Fluorescent Protein Guide

    Type
    Collection
    ...Lambert (Link opens in a new window) Nikon MicroscopyU (Link opens in a new window) Olympus Microscopy Resource... the applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing...many applications of fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein...Resource Center (Link opens in a new window) Leica Microsystems Resources for Microscopy in Life Sciences...Sciences (Link opens in a new window)...

  6. Plasmid Collections

    Type
    Collection
    ... part of a pathway or ORF. Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics...and depositor collections on one page to help you find what you're looking for. If you are looking for ...based on type or application. Browse our guides to find the plasmids and science information you need before...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established a common...

  7. AAV Molecular Tools

    Type
    Collection
    ...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and tetracycline... Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Viviana Gradinaru...transactivators that can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System...tTA) 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...separate plasmid that does so; however, these independent gRNA plasmids can be paired with a wide variety...empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can search ...Marraffini pgRNA-bacteria 44251 Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...Qi pRPR1(1xTetO)_gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI...pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156...60901 Mammalian U6 yes, FokI S. pyogenes Conklin pcrRNA.ind 61284 Bacteria araB none S. pyogenes Beisel pcrRNA.con...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information...

  11. CRISPR Plasmids - Yeast

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

  12. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  13. Resolute Plasmid Collection

    Type
    Collection
    ...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...RESOLUTE repository feedback form (Link opens in a new window) . If you have further questions or comments you...

  14. Brain Armamentarium

    Type
    Collection
    ...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

  15. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... each library can be found on the individual library page. Find Pooled Libraries of Interest Click...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout... Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout...234820 Knockout Mouse Ebert 3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast...

  16. CRISPR Plasmids - Plants

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization... fuse your protein to a red fluorescent protein (find plasmids for cloning in our Fluorescent Proteins...of interest. Browse or search the table below to find markers of general cellular organelles and structures...cell division. Browse or search the table below to find markers of cytoskeletal element. ID Plasmid Structure... desmosomes. Browse or search the table below to find markers for cellular junctions. ID Plasmid Structure...function properly. Browse or search the table below to find markers of secretory pathway components. ID Plasmid...

  18. CRISPR Plasmids - Tagging

    Type
    Collection
    ...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a...

  20. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...
Showing: 81 - 100 of 1083 results