We narrowed to 1,085 results for: IND

...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system that enables researchers... you first need to have a biological question in mind. Then you can decide how to answer that question... Cas9 exert their activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit...reverse transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be... choose from. Below are a few factors to keep in mind when designing and cloning your gRNAs. Select Genetic... editors exert their functions within a limited window from the PAM sequence, and base editor variants...variants often have different optimal editing windows. Be sure to refer to the literature associated with your...

...117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140135...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552...

...expression levels of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed along...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the second binds to mcherry...modulating expression levels of two targets independently. Depositor...plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage...

...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Figure 1: Overview of...large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized...

... optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...are large proteins made by the immune system that bind to an antigen, a particular part of a foreign substance...useful to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use...cells. Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example ... often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system... or ScFvs. Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP...

...COVID-19 Plasmid Collection (Link opens in a new window) Researchers developing novel diagnostics, therapeutics...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...early 2020, Ginkgo Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities...plasmids available to scientists in academic and industrial organizations. For more information on ordering...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or.... Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based ...(Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

... opens in a new window) Olympus Microscopy Resource Center (Link opens in a new window) Leica Microsystems... the applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing...many applications of fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent...proteins as well as hundreds of empty backbones. FRET Find FRET pairs and standards to study protein-protein... External Resources FPbase (Link opens in a new window) : an open-source, community-editable database ...Microscopy in Life Sciences (Link opens in a new window)...

...spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit... is truncated at both the N and C terminus and induces mutation at rates much higher than the parental...expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with strong nuclear localisation...TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...architecture has been shown to increase mutation induction when using TALENs. The FokI domains (DD, RR) used... protein (previously shown to preserve the DNA-binding ability of TALEs), 63 wild-type TAL amino acids...

... part of a pathway or ORF. Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics...and depositor collections on one page to help you find what you're looking for. If you are looking for ...based on type or application. Browse our guides to find the plasmids and science information you need before...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established a common...

...nature24031 (Link opens in a new window) PMID: 28980641 (Link opens in a new window) Davis L. & Chin J. W. (2012...nrm3286 (Link opens in a new window) PMID: 22334143 (Link opens in a new window) Shandell M. A., Tan Z., ....1c00286 (Link opens in a new window) PMID: 34196546 (Link opens in a new window) Credits Contributing Authors...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic...recruited to the ribosome, where its anticodon can bind with the complementary codon on the mRNA. If the...form an orthogonal pair, which means they work independently of the host cell's natural genetic machinery...the activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate...

...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...protein to a red fluorescent protein like mCherry (find plasmids for cloning in our Fluorescent Proteins...of interest. Browse or search the table below to find markers of general cellular organelles and structures...cell division. Browse or search the table below to find markers of cytoskeletal elements. ID Plasmid Structure...pCMU-MTUBr Microtubules (Plants) MAP4 microtubule-binding domain mCherry Maria Harrison *Fusions to other... desmosomes. Browse or search the table below to find markers for cellular junctions. ID Plasmid Structure...lysosomes, and more. Browse or search the table below to find markers for components of these membrane trafficking...

...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

...plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA ...identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized... organism by visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting...

...against 8,158 predicted protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA ...this page was generated with the help of Scott Lindner . Cut ID Plasmid Gene/Insert Selectable Marker ...large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized... organism by visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized... organism by visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting...

...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions....single plasmid, though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3...large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized... organism by visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting...

...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...