Skip to main content
Addgene
Showing: 81 - 100 of 836 results

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

  5. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode...making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's...

  6. Resolute Plasmid Collection

    Type
    Collection
    ...approximately 450 identified members. Many SLCs have mutations associated with disease and could become targets...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...or to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...solution through a 0.22 μm filter to remove any precipitates. Stain each well with 1 mL of 0.1% crystal violet...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...

  10. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...ZFNs) are effective tools for making targeted mutations in zebrafish. With support from the NIH and guidance...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...fragments can be used in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

  12. mTOR Pathway

    Type
    Collection
    ...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic...multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation...

  13. Pipetting Protocol

    Type
    Protocol
    ...Depending on the pipette you’re using, you will be rotating dials behind the numerical display or the top ...

  15. Viral Production

    Type
    Collection
    ...i.e., genome containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids...

  17. Western Blot

    Type
    Protocol
    ...Different SDS-PAGE gel tanks will have different gel orientations. Refer to the instructions for your specific...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...
Showing: 81 - 100 of 836 results