...This has also been identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts...tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain." PloS one 8.9 (2013):...PMID: 21102559. PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of ... Its Fruit Is Sweet The Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting...I’ll provide some tips that will help you think about how to get started with AAVs. Since my research ...understanding of receptor physiology and the effects of metabotropic and ionotropic influences on overall cell function... the biotechnology firm Biogen have recently collaborated to develop “AAV 3.0”, vectors that will have...

...encouraging mentees to pursue cool projects. Chapter 6: Projects in Wei Leong Chew's lab. Genome engineering...getting a lab up and running, and learn a little bit about what it was like for Wei Leong to work in George...

...the antibodies. Nature. 2015 May 21;521(7552):274-6. doi: 10.1038/521274a. PMID: 25993940. ​​Bradbury,...Reproducibility in Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165...Have you ever been stuck googling about an antibody that will be essential to your new research project...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and provide general...pattern should align with what the atlases (mentioned above) and literature suggest, providing an expectation...open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation by allowing scientists...

...process since May 2020. We find this process takes 4-6 weeks on average. Therefore, we recommend placing ...expedite the application process.  More information about export control licenses is available through the...Industry and Security. If you have any questions about this or other Addgene shipping and ordering processes...customer support team at [email protected].  Curious about how rabies plasmids are used in the lab? Check out...

...Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target sequence...endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

...fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent protein tools...of our educational resources. Listen to hear all about Professor Goedhart, his lab, and some of the tools...

...01015-5 As for target selection, we usually pick 3-6 guides around the region to find the most efficient... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology. Genome..., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

...previously filtered) Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 ...dilution to each well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus one 'no virus...transduction" by seeding 50,000 cells into each well of the 6-well dish. These cells will be added to the wells ...suspension (i.e., 50,000 cells) into each well of the 6-well dish. This brings the total volume in each well...expand into larger vessels. A confluent well of a 6-well dish can be expanded into a 10 cm dish. A confluent...cells in the untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor...

...importance of thinking about the big picture and not just the next experiment. Chapter 6: Advice to scientists...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

...by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA conjugates are ...These reagents are nonfluorescent until they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that ...Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...

...and green wavelengths, respectively, are selected (6). These previous blog posts help with the decision...Methods 14.1 (2017): 53-56. PubMed PMID: 27869816. 6. Media Cybernetics. Colocalization of Fluorescent ...the context of the cell. However, much knowledge about functionality can be gained by understanding the...subcellular marker proteins, it’s possible to get an idea about the subcellular structures your protein resides ...with. These “colocalization” studies give insights about the proximity of two proteins within the same subcellular...Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells...

...s41587-020-0491-6 Additional resources on the Addgene blog Find more blog posts about base editing Learn...ABE7.10. In addition to stem cells the authors targeted 6 genes in primary human T cells using 8 selected ABE8s...Learn about another evolved base editor: ABE8e Read about xCas9, a CRISPR variant with PAM flexibility...

...Activated by Designer Drugs. ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier... Conditions Rather than Specific Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO...posts Read blog posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our...as a DREADD agonist due to its ability to be metabolized into clozapine (CLZ). While some scientists argue...

...Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016):1777-85. PubMed PMID: 27172193. PubMed Central...tagged gene. Most insertions can only be detected by labor-intensive processes like PCR or by evaluating visual...repair after a CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been...Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable...

...Neurotropic Viruses. Curr Opin Neurobiol. 2008 Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, ...Neuroanat. 2015 Jul 1;9:80. Pubmed PMID 26190977. 6. Osakada F, Callaway EM. Design and generation of ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

...Phy–PIF Light-controlled Phytochrome Interacting Factor 6 (PIF6) to PhyB (Phy) interaction 660 Cryptochromes...targeted brain circuits. J Neurosci. 26(41):10380-6. PMID 17035522 Dugué GP, Akemann W, Knöpfel T. A comprehensive...transgene expression in neurons. Front Neural Circuits. 6:47. PMID 22866029 Wietek J, Beltramo R, Scanziani ...control derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

... CRISPR Plasmids by Addgene. The CRISPR Journal, 6(6), 493–501. https://doi.org/10.1089/crispr.2023.0059...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

...reaction will require 6 μL FuGENE® + 74 μL OPTI-MEM. For example: 1x master mix: 6 μL FuGENE® + 74 μL OPTI-MEM...community. Please cite Moffat et al., Cell 2006 Mar; 124(6):1283-98 (PubMed PMID 16564017 ) in all publications... Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Addgene #12259 HEK-293T cells GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001... Particles This protocol is for transfection in a 6 cm plate. The protocol can be scaled to produce different... plate 7×105 HEK-293T cells in 5 mL of media in a 6 cm tissue culture plate. Incubate cells at 37°C, 5%...viral production. d. Create a master mix of FuGENE® 6 transfection reagent in serum-free OPTI-MEM. Calculate...

...development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed Central...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org ...didn’t have to worry about having to hunt down sources later on.  A comment about writing: initially I...exists. Once you pick a topic, try to be specific about exactly what aspect of the field you plan to review...themes in the field and to learn what people cared about. I also used reviews to get a list of research papers...highlighted a few dozen things that I didn’t know about. Instead of reading a paper and getting my bearings...them. No really, just start writing! Don’t worry about grammar or formatting or continuity. Also don’t ...

... this is a beta project, it will run for at least 6 months as we assess the needs of the scientific community...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...scientific communities using plasmid tools. Details about the cloning grade DNA beta project We will ship ...quantify DNA Resources on Addgene.org Learn about our other beta projects Read our Molecular Biology Reference...