We narrowed to 363 results for: gne
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TypeBlog Post...been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...
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Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...Organoid EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated... heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...without end-point sequencing assays. This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post... of transcriptional regulator or the number of designed scaffold binding sites. With hundreds of characterized...targeted site. Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable to many schools...students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in outputs that ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...regulation of many genes. A number of groups have designed means of studying the effects of endogenous looping... they call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors... -
The MTA at Addgene
TypeBlog Post...organization is sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward ...plasmid hasn’t shipped because a form needs to be signed. But the truth is, meaningful progress depends ... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...a disease in a model system. TF screens can be designed to identify custom TF programs to acutely initiate...predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that initiates biological... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...than 45,000 volunteers from all over the world had signed up, and soon we started receiving dozens of requests...physics. Even non-scientists, such as lawyers, designers and other professionals, joined the platform; ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...directly to the original article online. It is also designed to be persistent and exist online indefinitely...deposit a plasmid with Addgene, your plasmid will be assigned an Addgene ID #, RRID, and resolvable compact ... -
Components of CRISPR/Cas9
TypeBlog Post...editing, researchers simply need to express a gRNA designed to direct Cas9 to their target sequence of choice... double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA. The “direct... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...necessary for studying endosomes, the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors...sgRNAs are used with FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post... RNA toehold switch, a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream...components for gene disruption or CRISPRi. While designed to study the bee microbiome, these broad-host-... -
Transferable Skills Guide: Public Speaking
TypeBlog Post... Own your time When I was an undergrad, I was assigned a paper to give as a journal club to my molecular...the student presenting right before me had been assigned a structure paper with two single-panel figures... -
All in a Twist: dsRNA
TypeBlog Post...also known as RNAi, are short, dsRNA molecules designed to degrade mRNA transcripts. RNAi activates the...sequence is found (typically RNA that the RNAi is designed against), RISC can repress translation and/or ... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...protein-coupled receptor, one of the Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Armbruster... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies...green-red Cre-lox systems. T-DDOGs can also be designed with other DNA-binding domains, including the ...