We narrowed to 413 results for: his

... pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available through Addgene. We have...Octet, Pall ForteBio). Tips for using pMal-T-Avi-His/BirA to add biotin to your protein of interest Grow...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins... the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see plasmid map and ...dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our proteins that...proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t find an all-in-one...

... Strategy at the MIT Sloan School of Management. His research focuses on a range of topics including biotech...and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/ .   References...

...Precision Genome Engineering and Synthetic Biology". His reflections on the program are here. This was a great...

...sciences. He also posted two preprints of his own research during his PhD studies.  References and Additional...

...As Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working...engineering projects in a very big way. In one of his latest papers, published in Molecular Systems Biology...regulation thats as sophisticated as a natural network. We could build a material as sophisticated as wood. To... carbons and oxygens. Nothing too much more sophisticated. There are also proteins, like antibodies. But...able to design new materials and chemicals as sophisticated as those that living cells and animals make....

...’re attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional...

...distributing his Designer Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids on his own, UNC-Chapel... risk of off-target effects. Harald Janovjak and his team at the Institute of Science and Technology Austria...Interested in purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and...Brainbow toolkit for neuronal imaging Joshua Sanes and his team at the Center for Brain Science at Harvard University...contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA ...

...in Chemistry for his contributions to super-resolution light microscopy, comes from his recent Nature Communications... super-resolution microscopy.” Endosomes are historically difficult to study in living cells using conventional...

...guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...publications (published, not in preparation) A history of successful funding (fellowship grants) A project...

... in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

...Faculty of Pharmacy at the University of Toronto. His research is focused on developing cell-free synthetic... Molecular Sciences at Arizona State University. His group carries out research in synthetic biology, ...

...supervisor’s management style, bring challenges to his or her attention as soon as you can. It is in your...you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for mutually...

...resources. Listen to hear all about Professor Goedhart, his lab, and some of the tools he’s developed.   Listen...

...Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's booth, #3422, in the nonprofits...

...Polytechnic Institute. He also studies energy markets in his spare time. You can follow him on twitter at @thompn4... error and at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles...Denver, CO, USA 39.7392°N 104.9903°W - Erin Golden Hi, my name is Erin and I have spent the last 15 years...

...Cruz where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also...conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter.   References Arribere...

...express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier...

...member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools...

...editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab at the University...developed a strong passion for computational biology. His expertise includes applying machine learning techniques...efficiencies, we could train our prediction model PRIDICT (Mathis & Allam et al., 2023). We trained our models in...training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in ...pegRNA editing efficiencies for specific targets (Mathis & Allam et al., 2023, 2024; Yu et al., 2023; Koeppel...variations in chromatin context. Our recent studies (Mathis et al., 2024) and concurrent research by the Shendure...Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A., Kissling, L., Marquart, K. F., ...

...Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität... Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab.     References D'Aoust...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...