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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 206 results for: icos

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Showing: 81 - 100 of 206 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sequencing data alongside the analysis of NGS-based amplicon sequencing data and found that the accuracy of...100-fold reduction in cost relative to NGS-based amplicon sequencing. To use the tool, you start by uploading...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...): aaf8729. PubMed PMID: 27492474. 3. Gaudelli, Nicole M., et al. "Programmable base editing of A• T to...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...polypeptide chain. One part of the tRNA contains the anticodon,  a three nucleotide sequence complementary to... binds the amino acid that corresponds to the anticodon sequence. That means there are 61 possible tRNAs...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... 1), in China, Korea, Japan, Brazil, India, and Mexico. Table 2: Addgene distributors Distributor ...2015 Korea San Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences 2020 India ...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy...Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense ...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ..., to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine... of bright luminescent protein for real-time multicolour bioimaging. Nature Comm. 7, 13718. PubMed PMID...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... post was written by Alyssa Shepard and Angelo Nicolaci, a PhD student at Moffitt Cancer Center. Engineering...end_module_attribute %}{% end_module_block %} Angelo Nicolaci is a PhD candidate in the lab of Jennifer Binning...

  9. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim C, Boulanger CM (2012) ...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...kits for plants that work for both monocots and dicots (Hahn et al., 2020; Chamness et al., 2023; Stuttmann... Multiplex Editing: One‐shot Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The ...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... a powerful tool to modify mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru... School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous...2015. PubMed PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Alexis Komor and the first adenine base editors from Nicole Gaudelli in David Liu’s lab in 2016. History of...al., 2025). Adenine base editors The beginnings Nicole Gaudelli from David Liu’s lab created an adenine...

  15. Chemogenetics Plasmids

    Type
    Collection
    ... KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond...

  16. Synthetic Biology - Overview

    Type
    Collection
    ...Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins...

  18. mTOR Pathway

    Type
    Collection
    ...independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources ...

  19. Microbiology Resources

    Type
    Collection
    ...Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium ...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism...
Showing: 81 - 100 of 206 results