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Showing: 81 - 100 of 205 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... 1), in China, Korea, Japan, Brazil, India, and Mexico. Table 2: Addgene distributors Distributor ...2015 Korea San Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences 2020 India ...

  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...polypeptide chain. One part of the tRNA contains the anticodon,  a three nucleotide sequence complementary to... binds the amino acid that corresponds to the anticodon sequence. That means there are 61 possible tRNAs...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...): aaf8729. PubMed PMID: 27492474. 3. Gaudelli, Nicole M., et al. "Programmable base editing of A• T to...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...antibodies, making them difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP...specific reactivities making them more useful for multicolor imaging experiments than standard FPs. The Looger...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sequencing data alongside the analysis of NGS-based amplicon sequencing data and found that the accuracy of...100-fold reduction in cost relative to NGS-based amplicon sequencing. To use the tool, you start by uploading...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Streptomyces , and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...is critical that the deletion and non-deletion amplicons be easily resolved on an agarose gel for multiplexing... with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into a plasmid...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy...Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense ...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ..., to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine... of bright luminescent protein for real-time multicolour bioimaging. Nature Comm. 7, 13718. PubMed PMID...

  11. Bacterial Expression Systems

    Type
    Collection
    ...terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX expression vector with N-terminal...purification from the Structural Genomics Consortium and Nicola Burgess Brown containing a variety of tags can ...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter Description...Firefly EF1α AAV expression of firefly luciferase Nicole Paulk 98294 pF CAG luc IRES neo Firefly CAG Lentiviral...

  13. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim C, Boulanger CM (2012) ...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...kits for plants that work for both monocots and dicots (Hahn et al., 2020; Chamness et al., 2023; Stuttmann... Multiplex Editing: One‐shot Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The ...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... a powerful tool to modify mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru... School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous...2015. PubMed PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Alexis Komor and the first adenine base editors from Nicole Gaudelli in David Liu’s lab in 2016. History of...deaminases. Adenine base editors The beginnings Nicole Gaudelli from David Liu’s lab created an adenine...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...transformation. Addgene depositors Sylvestre Marillonnet and Nicola Patron have assembled two collections of standardized... 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...

  20. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting ...
Showing: 81 - 100 of 205 results