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We narrowed to 760 results for: mpo

Showing: 81 - 100 of 760 results

  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select...in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select the Insert...

  2. Kit Free RNA Extraction

    Type
    Protocol
    ...Air-dry the pellet for 5-10 minutes. Critical It is important to not let the pellet get too dry before resuspending...

  3. DNA Quantification

    Type
    Protocol
    ... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

  7. Video Library

    Type
    Protocol
    ... office, discusses her career in science, the importance of international collaboration, and offers career...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

  11. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...plasmid. It shows you each component, with the ID of the component plasmid, component name, size, and any comments...module, are the components. Using the handy website, users can simply select the components they want for...able to select components that work with your design. Note that you can browse all components by clicking...Fragmid doesn’t have the components I want? You can make your own Fragmid components! Ensure your backbone... Each module of Fragmid represents a class of components, such as your CRISPR enzyme or Pol 2 promoter...’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything...everything to stay in the correct order. Most Fragmid components are available for request on the Addgene website...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...different components, but all contain a FRET pair, a genetic component, and a small molecule component. Early...developed voltage sensor with a genetic component. The VSD components have historically come from a variety...cellular localization component is typically handled by either the fluorescent component (i.e., when the dye...tools have two main elements: a voltage-sensing component and a fluorescent output. The GEVI family of gadgets... a hybrid approach and utilize both a genetic component as well as a small molecule which we will describe...reporter and the mechanism of their voltage sensing component, which separates them into two distinct categories...squirts. This design structurally separates the two components of the indicator, which can make the kinetics...

  13. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...replication components are called helper plasmids. Below, we’ll breakdown the vector components first by ...nucleocapsid viral components needed for viral packaging, assembly, and display. Pol, the last component, contains...there are bonus components to these vectors that aren’t reflected in the common components outlined above...lentiviral guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...production reasons, multiple plasmids with many components are used to package lab grown viruses. It can... protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their... what gag and pol stand for, we demystify all components of viral vectors here! Need Virus? Check out...

  14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...compatible with all your components. Next, consider the best form for those CRISPR components based on your model...introducing those CRISPR components into your target cells. Delivery of these components into mammalian cell...most effectively achieved with different CRISPR components. Select the appropriate CRISPR tools for your...will you be editing? Do you want to deliver the components as DNA, or would mRNA or protein delivery be ...of the first steps is to identify what CRISPR components you will need to deliver. Minimally, one or more... the best method for introducing those CRISPR components into your target cells.  Mammalian CRISPR expression...best practices for efficient delivery of CRISPR components. If you are new to CRISPR or your model system...

  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...Advocating for Science Symposium Boston 2016 Changes since the Future of Research Symposia Ongoing challenges...skills. Advocating for Science Symposium and Workshop 2016 At past symposia, FoR worked to highlight some...necessary for affecting change. On Friday, the symposium includes a panel discussion and keynote by former...eLife, 2016). Several Future of Research (FoR) symposia held across the USA during this time have revealed...the efficacy of their advocacy skills. Friday’s symposium will include a discussion on effective advocacy...Chief Diversity Officer Harvard University  The symposium will culminate with a keynote address by former...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ... of 10 mL, we’ll want 200 mL of media total. Importantly, we’ll actually make a bit more than 200 mL (...

  17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ... whether respondents saw them as not important to very important. In addition, we also explored the barriers... study, respondents were also asked about how important ease of use is for them (if they are to switch...ELN choice, with almost 80% rating it as very important. One comment reflected the desire for a flexible... category;”. The study also showed that It is important that an ELN supports all types of data, which ...data during their work. It is already becoming impossible to keep track of it all in paper notebooks. Following...supplement their paper notebooks. Instead, all of the important information is consolidated to one place, easily...paper records as well as electronic ones? It is important to know in which ways your data can be exported...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...sequence are important considerations for designing your gRNAs. For indels, it's not so important what location...nom. Me love gRNAs! Comic by Maya Kostman.   Important considerations before you start an experiment ...tool among them. While this seems obvious, it is important to remember that the same is true when designing...location in the gene you target, but it is important that your gRNA sequence is designed to be highly active...CRISPRi, these considerations are of roughly equal importance (target should be near the TSS but you can worry...from). Finally, for HDR, location is much more important because you have to target within ~30 nt of your...gRNA with an optimized sequence is of primary importance (more on this below). Created with BioRender.com...

  19. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...proteins are provided in trans (see Other Important Components of EBOV Minigenome System). However, minigenomes...sense RNA viruses (reviewed in 2, 3). Other important components of the Zaire ebola virus minigenome system...antiviral compounds which often target the viral polymerase. Technical tips It is important that all of...particular observation can be easily studied as each component can be examined independently. In addition, experiments...efficiently transfected into cells. All of these components are necessary to efficiently reconstitute a functional...utilize the minigenome as a template. Thus, it is important to choose cell lines that are highly transfectable...plasmid transfected into cells are also extremely important. For example, particular ratios of plasmids encoding...

  20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...interacting proteins. No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “...Split-BioID, a spatiotemporally controllable version of the BioID protocol. The critical component for both ... lysate fractionation. Important considerations for Split-BioID It’s important to remember that with Split-BioID...proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nature communications...this limitation by creating Split-BioID, a spatiotemporally controllable version of the proximity-dependent... other PPI methods, including BioID, is its spatiotemporal controlled labeling of interacting proteins...protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly interact...
Showing: 81 - 100 of 760 results