...Conclusion Pipetting accurately and precisely is important since even small changes in the volume could affect...Beyond knowing the proper way to pipette, it’s important for scientists to calibrate their pipettes to ...

...organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian...vivo . The iodixanol gradient in this protocol is composed of steps that separate out contaminants from an...

...of cell death upon antibiotic selection. It is important to monitor these cells regularly and replace the... the surviving cells in the culture, so it is important to do regular media changes and maintain optimal...

...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select...in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select the Insert...

...Air-dry the pellet for 5-10 minutes. Critical It is important to not let the pellet get too dry before resuspending...

... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...

...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

...the safest way possible. Protecting yourself is important! Disclaimer: The SOP presented here has been designed...

...that you have is the expected size, or that it is composed of a backbone and insert of expected sizes. This...

... office, discusses her career in science, the importance of international collaboration, and offers career...

...Protecting yourself and working smart in the lab is important! Disclaimer: The SOP presented here has been designed...

...Master Mix" consisting of all of the reaction components except for the DNA. Aliquot your DNA into individual...

... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

...plasmid. It shows you each component, with the ID of the component plasmid, component name, size, and any comments...module, are the components. Using the handy website, users can simply select the components they want for...able to select components that work with your design. Note that you can browse all components by clicking...Fragmid doesn’t have the components I want? You can make your own Fragmid components! Ensure your backbone... Each module of Fragmid represents a class of components, such as your CRISPR enzyme or Pol 2 promoter...’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything...everything to stay in the correct order. Most Fragmid components are available for request on the Addgene website...

...different components, but all contain a FRET pair, a genetic component, and a small molecule component. Early...developed voltage sensor with a genetic component. The VSD components have historically come from a variety...cellular localization component is typically handled by either the fluorescent component (i.e., when the dye...tools have two main elements: a voltage-sensing component and a fluorescent output. The GEVI family of gadgets... a hybrid approach and utilize both a genetic component as well as a small molecule which we will describe...reporter and the mechanism of their voltage sensing component, which separates them into two distinct categories...squirts. This design structurally separates the two components of the indicator, which can make the kinetics...

...replication components are called helper plasmids. Below, we’ll breakdown the vector components first by ...nucleocapsid viral components needed for viral packaging, assembly, and display. Pol, the last component, contains...there are bonus components to these vectors that aren’t reflected in the common components outlined above...lentiviral guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...production reasons, multiple plasmids with many components are used to package lab grown viruses. It can... protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their... what gag and pol stand for, we demystify all components of viral vectors here! Need Virus? Check out...

...compatible with all your components. Next, consider the best form for those CRISPR components based on your model...introducing those CRISPR components into your target cells. Delivery of these components into mammalian cell...most effectively achieved with different CRISPR components. Select the appropriate CRISPR tools for your...will you be editing? Do you want to deliver the components as DNA, or would mRNA or protein delivery be ...of the first steps is to identify what CRISPR components you will need to deliver. Minimally, one or more... the best method for introducing those CRISPR components into your target cells.  Mammalian CRISPR expression...best practices for efficient delivery of CRISPR components. If you are new to CRISPR or your model system...

...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...Advocating for Science Symposium Boston 2016 Changes since the Future of Research Symposia Ongoing challenges...skills. Advocating for Science Symposium and Workshop 2016 At past symposia, FoR worked to highlight some...necessary for affecting change. On Friday, the symposium includes a panel discussion and keynote by former...eLife, 2016). Several Future of Research (FoR) symposia held across the USA during this time have revealed...the efficacy of their advocacy skills. Friday’s symposium will include a discussion on effective advocacy...Chief Diversity Officer Harvard University  The symposium will culminate with a keynote address by former...

... whether respondents saw them as not important to very important. In addition, we also explored the barriers... study, respondents were also asked about how important ease of use is for them (if they are to switch...ELN choice, with almost 80% rating it as very important. One comment reflected the desire for a flexible... category;”. The study also showed that It is important that an ELN supports all types of data, which ...data during their work. It is already becoming impossible to keep track of it all in paper notebooks. Following...supplement their paper notebooks. Instead, all of the important information is consolidated to one place, easily...paper records as well as electronic ones? It is important to know in which ways your data can be exported...

...sequence are important considerations for designing your gRNAs. For indels, it's not so important what location...nom. Me love gRNAs! Comic by Maya Kostman.   Important considerations before you start an experiment ...tool among them. While this seems obvious, it is important to remember that the same is true when designing...location in the gene you target, but it is important that your gRNA sequence is designed to be highly active...CRISPRi, these considerations are of roughly equal importance (target should be near the TSS but you can worry...from). Finally, for HDR, location is much more important because you have to target within ~30 nt of your...gRNA with an optimized sequence is of primary importance (more on this below). Created with BioRender.com...