Zhang Lab's CRISPR Frequently Asked Questions
Type
Collection
... discriminate between positive and negative clones. Clones with insertion will show only linearized plasmid...FAQs Can you summarize HR template vector design guidelines? A few notes below are considerations for designing...plasmid of ~8.5 kb (only AgeI will be able to cut). Clones without insertion will show a ~1kb and ~7.5kb fragment...) on each colony in order to screen for positive ones. - Or - If your HR template has a selection marker... primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...