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We narrowed to 913 results for: nes

Showing: 81 - 100 of 913 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...in between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl...acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171 Partially...  Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  pCSW21  Yes...quickly and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative diseases...compatible cloning sites for easy cloning of your genes of interest. In order to improve the study of specific...induction of structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...

  2. Plan Your Experiment

    Type
    Collection
    ...more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression...can be transient, or you can generate stable cell lines if your plasmid contains a selection marker. Plasmid..., note that this method is most useful for cell lines that you can transfect at high efficiency, such ... are a popular option for producing stable cell lines, since the genetic cargo (in this case, CRISPR components...is a common choice for generating modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA...Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use the ...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This...be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the following...

  4. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...fluorescent iRFP variants have been shown to have high brightness and allow for single or multicolor imaging. Photo-activatable...blog post . Far Red Protein Excitation/Emission Brightness Plasmids mCardinal 604/659 18 mCardinal plasmids...Crimson Near-Infrared Protein Excitation/Emission Brightness Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670... Photoactivatable Protein Excitation/Emission Brightness Plasmids PAiRFP1 690/717 (after photoactivation...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications to the the pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...E., Schneider, S. R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski, J. A. (2021). Initiation of...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...

  6. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate...diverse set of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

  7. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs...):1471-1501. PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases...

  8. p53 Pathway

    Type
    Collection
    ...in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid... phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper...

  9. TALEN Guide

    Type
    Collection
    ... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...di-residue (RVD). Ultimately, it is this RVD that determines which single nucleotide the TAL effector will... Daniel Voytas’s group from the University of Minnesota, arrays can be assembled in a matter of days. ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove...

  10. Arf GTPase Family

    Type
    Collection
    ...GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene...list of plasmids available. Please note that some genes may not yet have plasmids available for request.... GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each...

  11. Open Enzyme Collection

    Type
    Collection
    ... Depositor Collections FreeGenes Project Open Enzyme Collection FreeGenes Project Open Enzyme Collection...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of ...Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification...

  12. Lentivirus Plasmids

    Type
    Collection
    ...Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation, two plasmids ...selection. See Root Lab pLEX and pLIX Gateway backbones for additional variants. David Root 85140 pLV-...expression of hTERT, used to create immortalized cell lines. See Hayer et al. (2016) for additional selection...See Bloemberg et al. (2020) for additional CAR backbones. Scott McComb 145025 TFORF3549 3rd For barcoding...

  13. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...single nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets...The following plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate...

  14. KLF Research Plasmids

    Type
    Collection
    ...Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...Addgene or email [email protected] to get started. Genes KLF gene pages with information on available plasmids...

  15. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...images, tutorials, protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue...

  16. CRISPR Plasmids - Parasites

    Type
    Collection
    ...(toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and ...10 guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that...

  17. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ...

  18. Promega Plasmid Collection

    Type
    Collection
    ... DNA size are processed and shipped within 6–8 business days. For more information about the ordering ... form an active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT... detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT...

  19. Ras Pathway

    Type
    Collection
    ...a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam K, Schreck...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD ...mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction ... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...
Showing: 81 - 100 of 913 results