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  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...unexpectedly failed to produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was...expression constructs. The Trimmer lab found that the recognition sequence for the restriction enzyme BciVI was...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... to cleave 4 gRNAs separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although...only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility indicates...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... tagged with a Spaghetti Monster because they recognize this GFP scaffold itself. Fortunately the GFP1...GFP1 HA Spaghetti Monster RANbody doesn’t recognize the scaffold and is useful for detecting GFP. Spaghetti...

  6. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... have to worry about and that is something to recognize! Distributing plasmids, viral service, and more...every day in every way. Scientists know this and recognize it all the time. We love this feedback and are...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...to its target. While most secondary antibodies recognize broad isotype classes, it is possible to get secondary...multiplexed assays, using secondary antibodies which recognize specific isotype subclasses of primary antibodies...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ...that arise during transcription are typically recognized by RNase H which hydrolyzes the RNA portion, ...Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: ...

  9. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...  Sugar phosphate backbone modifications The recognition sequence for CRISPR-Cas9 targeting is led by ...cytoplasmic and nuclear nucleases, as they will be recognized as foreign RNA. To protect the RNA from degradation...

  12. MXS Chaining

    Type
    Blog Post
    ...techniques are free of specific restriction enzyme recognition sites. Thus, chaining methods are not necessarily...1961, Swartz et al., 1962), SalI, XhoI and MluI recognition sites, which contain CpG dinucleotides in their...

  14. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...until either the PAM site or part of the guide’s recognition sequence is destroyed via mutation. By introducing...introducing a mutation in either the PAM or the guide recognition sequence in the donor DNA, you can guarantee ...

  16. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they have created antigenic...imaging set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with...

  17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now referred to as Cas13a... or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as specified...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...the mutant versions of SpCas9 and SaCas9, which recognize PAM sequences other than NGG (Table-1). Type...or destruction of any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used...
Showing: 81 - 100 of 258 results