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Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Protocols for plasmid cloning by PCR.

Learn about flow cytometry labeling strategies beyond extracellular markers in this Antibodies 101 post!

Model organisms are species that are widely studied because they are easy to maintain and modify in the lab. Common model organisms include the mouse, the fruit fly, yeast, zebrafish, and the worm.

The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience research. Find plasmids encoding these R-mAbs here.

Learn about new tools in Addgene's collection!

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, gives advice on how to complete a successful postdoc and set yourself on a path to a successful academic career.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

Find some tips and tricks for using CRISPR for gene editing.

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.