...abundance, and thus difficult to purify in quantities necessary for study. Moreover, the ability to isolate purified...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified ...interest as possible, some optimization will be necessary. To begin, one should optimize protein production...

...enough funds to get started, wasting funds on unnecessary materials, or even a loss of the offer all together...usually focus on the numbers, rather than the necessary resources. This is why at Experimental Designs...too often, we see early investigators making unnecessary short term sacrifices that hurt their goals, ...

...reproducibility. For a study to be “reproducible” does not necessarily mean that someone has replicated it precisely...them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers of pre-registered...practices including talking to statisticians when necessary and encouraging interlab peer review of experimental...

...and validation Can’t forget the controls! The necessary control experiments will vary depending on your...standard IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  ...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...withholding necessary information Overwork – including undue pressure, impossible deadlines, unnecessary disruptions...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...FRET acceptor labeling specificity is not always necessary for successful FRET experiments. If the acceptor...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have...When expression plasmids are available, or the necessary expertise to clone them, it can be useful to produce...to find for many proteins. In practice, it was necessary for us to combine overexpression with the use ...

...Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed...

...bad mentor. Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors...developing colleagues even as I reinforce the necessary power dynamic between advisor and students.” Stay...

... special year for Addgene: it marks our 20th anniversary. Throughout the year, we’ll be celebrating with... You can enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social...

...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole circuits... circuits or tissue-wide systems is necessary, use chemogenetics. Note that, as with all tools, both approaches...

...scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-intensive...Have their own working schedules and may not necessarily be comfortable with imposed deadlines (there’...

...uncertainty and precautionary regulation was all necessary to inform the experimental aspect of my thesis...its own right, science communication reflects a necessary confluence of science and the world it hopes to...

.... The double stranding step was assumed to be necessary because the AAV genome is single-stranded and ...this double stranding step might not actually be necessary. Since the AAV genome exists as [+] and [-] strands...

...pending legal agreement ever delayed your access to necessary research materials? If so, you should always talk...Whitehead Institute for Biomedical Research Glossary Master Deposit Agreement: An agreement, executed...

...fibers would need to be, as well as other features necessary for implantation in brain tissue. The gut, on...relatively small, and can be detached easily whenever necessary. This is all an impressive engineering feat, ...

...believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect, chocolate-covered...connections on a regular basis. It is also not at all necessary. Once you’ve talked with a person a few times,...

...interactions and #social support are even more necessary during these times of stress due to #COVIDー19.... virtually. With co-working, the idea is not necessarily to chat, but to mimic your usual work environment...

... at times."     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000...Following history from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five...

...higher titer than others. High titers are often necessary to visualize expression of fluorescent proteins... get a good fluorescent signal. It may not be necessary to concentrate this type of vector to such a high...