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...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic...multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation...

...i.e., genome containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...83R] BAF53a Human Mouse IgG2a 206558 Anti-ATAT1 [N347/42R] ATAT1 Human Mouse IgG2a 206559 Anti-Foxi2 [N349...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...

...Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification Measure DNA concentration...

...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

...colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °C Water bath at 42 °C Ice bucket...

...solution through a 0.22 μm filter to remove any precipitates. Stain each well with 1 mL of 0.1% crystal violet...

...Depending on the pipette you’re using, you will be rotating dials behind the numerical display or the top ...

...incubator. Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-transfection, supplement...

...Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana...Service : Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

...cut the bottom of the Gravitrap column at the indentation in the tip. Equilibrate the column with 10 mL...

...Different SDS-PAGE gel tanks will have different gel orientations. Refer to the instructions for your specific...

... before any wells display a green product. A precipitated product indicates the presence of too much HRP...

...Sample Calculation First, use a hemocytometer to quantitate the homogenized cell solution. Homogenized cell...

...Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

...Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...