We narrowed to 1,149 results for: Gene
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TypeBlog Post...Posts Last month there was plenty of excitement at Addgene. Our new viral service has begun to pick up steam... Lego Movie). October was no less busy for the Addgene blog which once again had record readership with...
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Troubleshooting and Optimizing a Western Blot
TypeBlog Post...resources on the Addgene blog Technical Design of a Western Blot More resources on addgene.org ...spend optimizing it. Blots for quantification generally require more optimization than blots used to confirm... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype... -
9 Tips to Achieve Success in Academia
TypeBlog Post...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ... -
Fostering communication in remote collaborations
TypeBlog Post...which problems can be addressed and new ideas generated. Furthermore, this casual communication reflects...start something new! Additional resources on the Addgene blog Read our blog post about external collaborations... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...controlling the amount of cutting and mitigating noise generated by off target digestion. After MNase digestion...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...DOI:10.1126/sciadv.abl9171 More resources on the Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies... -
10 Ways to Share Your Science!
TypeBlog Post...their work and their interest in science more generally to people outside of academia. Here are my recommendations...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on...sport-related concussion. More resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...those ideas into commercial products and next generation companies. We launched in the early spring of...Additional Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio... -
CRISPR References and Information
TypeCollection...identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9...CRISPR screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling...sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support...target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Includes a wide range... to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... -
MAPK Plasmids
TypeCollection...deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...score (Mattei et.al., 2022). Figure 1: Generation of immunogenicity scores by predictive algorithms...and current Scientist, DNA Sequencing and QC at Addgene. References and resources References Mosch, ... -
Components of CRISPR/Cas9
TypeBlog Post... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...set of proteins the green and red proteins are generally the most detectable and so should be used to tag... -
Advice for Scientists Starting a Lab
TypeBlog Post...studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for ...Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...helpful changes are endless. Fig. 2: Blugene moves reagents from left to right as they use them...documents/Western%20Blotting.pdf More resources on Addgene’s blog A Guide to Getting Started in Undergrad ... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...stellar resume and cover letter can be found on the Addgene blog. 3. Personal statement brilliance In addition...co-founder of STEAMid. Additional resources on the Addgene blog Check out other tools and resources for early... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot... first meeting and get organized. Discuss your general areas of interest and what you want to cover during...