We narrowed to 1,147 results for: Gene

...this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot chocolate...

...EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com...holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus...

...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for...tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting high-throughput...Promega — now available through Addgene. As a purpose-driven nonprofit, Addgene offers expert support, educational...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...subunits come together to form an active enzyme and generate a bright luminescent signal in the presence of...window) blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and...

...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...

...Posts Last month there was plenty of excitement at Addgene. Our new viral service has begun to pick up steam... Lego Movie). October was no less busy for the Addgene blog which once again had record readership with...

...table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication ...cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research...against p53 in different gene/insert combinations A more efficient method to generate integration-free human...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...S1934-5909(19)30423-0. Schöler You can also find gene-specific plasmids at the following links: NANOG ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...

...resources on the Addgene blog Technical Design of a Western Blot   More resources on addgene.org ...spend optimizing it. Blots for quantification generally require more optimization than blots used to confirm...

.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...

... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

...controlling the amount of cutting and mitigating noise generated by off target digestion. After MNase digestion...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

...which problems can be addressed and new ideas generated. Furthermore, this casual communication reflects...start something new! Additional resources on the Addgene blog Read our blog post about external collaborations...

...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...DOI:10.1126/sciadv.abl9171 More resources on the Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies...

...their work and their interest in science more generally to people outside of academia. Here are my recommendations...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your...

...video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on...sport-related concussion.   More resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer...

...those ideas into commercial products and next generation companies.  We launched in the early spring of...Additional Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...and regulatory genes; one plasmid in second-generation, two plasmids in third-generation Envelope plasmid...contains packaging gene env This collection highlights popular second- and third-generation lentiviral packaging...chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Garry...pULTRA 3rd Bicistronic expression of EGFP and the gene of interest. Can also express shRNA (cloning H1-...Modification of pLL3.7 with added genetic elements known to prevent epigenetic silencing. Expresses shRNA under...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...stably into the host genome, enabling long-term transgene expression. Wild-type lentiviruses have been engineered...

...score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores by predictive algorithms...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...

... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...