CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
Type
Blog Post
...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post.
Additional Resources on the Addgene Blog
Learn about Homology Directed Repair
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Resources on Addgene.org
Check out CRISPR Topic Page
Find CRISPR Nickase...