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  1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...
  2. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...
  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...further consolidate these FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the ...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...
  4. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...not permeate through your tissue samples as homogeneously as a single antibody. Using a secondary antibody...10.1177/38.7.2355173  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...
  5. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science writing, ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers...AP biology classes Creating a podcast about genetics using dog breeds as an example adults 45-55 yrs... you next time!  Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication...
  6. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...need to get there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How ...
  7. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...The contents of this post are intended to convey general information only and not to provide legal advice...you think about your career (and your future in general), keep in mind that you may have years to work ...www.casseuslaw.com.    Additional Resources on the Addgene Blog Transferable Skills Guide 6 Steps to Submitting...
  8. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...
  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... [the] academic workforce is pyramidal/feudal generating too many trainees per PI” “Lack of awareness ...Dinman, Chair of the Cell Biology and Molecular Genetics Department at University of Maryland College Park...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...
  10. Design Tips for Prime Editing

    Type
    Blog Post
    ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...
  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ....1371/journal.pone.0038313 More resources from Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies...
  12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...study binary protein interactions. However, FRET generally only tells you if two proteins interact and cannot...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...
  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages...
  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Gelbart, PhD - Director of Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...
  15. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...and Success in Management Resources at Addgene.org Addgene's Management Team ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Business School Blog Additional Resources on the Addgene Blog Management for Scientists Series What Makes...
  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...
  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...obscures the flexible nature of all proteins in general, and the amazing conformational flexibility that...classroom or trying to explain your science to the general public, visit the Center for BioMolecular Modeling...Molecular Designs.     Additional Resources on the Addgene Blog Learn All about CRISPR Use CRISPR for Genome...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...
  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...
  19. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...them onto the gel. The figure above summarizes generalized expected results for the three primers previously...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...
Showing: 1001 - 1020 of 1138 results