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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: LEA

Showing: 1001 - 1018 of 1018 results

  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the... existing software (a quick internet search will lead you to (Link opens in a new window) here and many...therefore it is important that the digest goes at least 4 hours and as long as overnight. If you are going...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...should be added upstream in order for the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your ...Protocol Video Watch the protocol video below to learn how to design primers for PCR. Reference Page Top...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ...Lentivirus is generally considered biosafety level 2+. Please ensure that you are in compliance with your institution...counting cells with multiple integration events leading to underestimation of the true titer. Calculate...

  5. General Transfection

    Type
    Protocol
    ...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric ... the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

  6. Lentivirus Production

    Type
    Protocol
    ...slowly add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric...the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

  7. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Protocol Video Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting...doing diagnostic digests and how to interpret them please see the Diagnostic Digest page. Purifying DNA from...

  8. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...Protocol Video Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). ...check out our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What...

  9. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...similar outcomes when using these protocols. However, please be aware that the protocol may need to be adjusted...conical tubes, VWR 89039-656 96-well polyester (clear) microplate seal, Thermo Scientific 5701 Microcentrifuge...

  10. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Selective pressure on this heterogeneous cell pool could lead to reduced transgene expression over time, as the...individual cells, which can be difficult to find. Leave the cells undisturbed in an incubator for 7–14 days...

  15. Immunocytochemistry

    Type
    Protocol
    ...similar outcomes when using these protocols. However, please be aware that the protocol may need to be adjusted...

  16. Colony Formation Titering Assay

    Type
    Protocol
    ...disturb the colonies. Count the colonies for at least 2 of the dilutions. Pro-Tip The higher dilution ...
Showing: 1001 - 1018 of 1018 results