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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,035 results for: cat

Showing: 1001 - 1020 of 1035 results

  1. Pipetting Protocol

    Type
    Protocol
    ...dispensed. The boxes that the tips come in often indicate a volume range that the tip can hold. This should...no liquid should drip from the tip. This could indicate that the tip is not on the pipette properly. Place...pipette tip by holding the pipette tip over your dedicated waste container and pressing on the tip ejector...

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...that the transgene has integrated in different locations in the various cells in the culture. This is because...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...test results. Sample Data In all cases below (-) indicates that the tested strain is not supposed to be resistant... resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the ...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...primers produce inaccurate, nonspecific DNA amplification product, and long primers result in a slower...which creates primer dimers and disrupts the amplification process. When designing, if unsure about what...

  6. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45...

  7. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... room. Make sure that you know where these are located before you start. Use the eyewash station if unwanted...choose to reuse or repurpose this SOP in another location, please note that you do so at your own risk; ...

  8. Immunocytochemistry

    Type
    Protocol
    ...Guide Western Blot Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry is...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  9. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...

  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide Introduction This protocol...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize...

  11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) Trypsin...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...

  12. AAV ddPCR Titration

    Type
    Protocol
    ...post on ddPCR for AAV quantification. This protocol was modified from the publication Lock et al. (2014) ...

  14. Weighing Reagents Protocol

    Type
    Protocol
    ...in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before ...

  15. Water Bath Protocol

    Type
    Protocol
    ...bring your materials to a particular temperature, catalyze chemical reactions such as restriction digests...

  16. Centrifugation

    Type
    Protocol
    ...centrifuge begins to wobble or shake, this could indicate an unbalanced or improperly loaded centrifuge....

  18. Gibson Assembly Protocol

    Type
    Protocol
    ...product, gel-purify DNA segments. Otherwise, PCR purification or even the raw PCR mix can work fine in an ...

  19. General Transfection

    Type
    Protocol
    ...inhibit transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For...

  20. CRISPR Guide

    Type
    Guide
    ... organisms, but modifications to various Cas enzymes have extended CRISPR applications to increasingly...following the amplification protocol specified by the depositing lab. After amplification, you’ll need ...heritability. Not all modifications are stable across cell divisions, and some types of modifications are more frequently... subsequently isolated by affinity purification. After purification, molecules associated with the locus...conventional methods for genomic purification, CRISPR-based purification methods are more straightforward...and SHERLOCKv2) allows for greater amplification and quantification and can be used on lateral flow strips...table above, 3' and 5' indicate on which end of targeted sequence the PAM is located. The majority of the...
Showing: 1001 - 1020 of 1035 results