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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: Kin

Showing: 51 - 60 of 938 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 ...Knockout Human Doench and Root 3rd 4 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout...(Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang... Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 ...Library 140633 Knockout Human Wei 3rd 3 58,630 Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout...

  2. Promega Plasmid Collection

    Type
    Collection
    ...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins....analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...presence of a substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  4. Antibody Guide

    Type
    Collection
    ...important factor (for instance, looking for a different conjugate or looking for antibodies validated for...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ... assay, called native ChIP, does not use cross-linking and instead relies on strong interactions between...the product information for similar antibodies, looking for ones that have identical features and epitopes...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

  7. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...experiments and can be used as positive controls when working out a new protocol. Read more about how to design... the cell type. Before proceeding, we recommend asking labmates/colleagues, searching the literature, ...efficiency. Edit Type The type of edit you are looking for will ultimately depend on which CRISPR method...

  8. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  9. Distribution to Industry

    Type
    Collection
    ...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don't See What You're Looking For? Please let us know what you would like to ...

  10. Genetic Code Expansion

    Type
    Collection
    ...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...
Showing: 51 - 60 of 938 results