We narrowed to 1,049 results for: ins

...Creating Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! ...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...the Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected ...

...of light hitting the sample or b) using dyes or stains that add color to some components of the sample..., be sure to record the magnification used, any stains used in your sample, and sample information so ...future. Conclusion Like any important laboratory instrument, you should be sure to take care of your microscope...

...should be properly disposed of following your institution’s hazardous waste procedures. Last Update: February...February 7, 2022 Protocol Video Watch this instructional video to learn how to affinity purify recombinant...for your columns, refer to the manufacturer’s instructions. Attach the Gravitrap column to a clamp on a...

... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

...of plasmids in your purified DNA. This protocol explains how to isolate a single bacterial colony by streaking...plasmids or bacteria need to be grown at 30 °C instead of 37 °C. This is often true for large unstable...

...Envelope plasmid, and Transfer plasmid encoding your insert of interest Reagent Preparation DMEM Complete (... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...

...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February... °C. See below for other Reagent Preparation instructions. Wipe down all pipettes and equipment with 10%...

...purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol ...of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image adapted...

...cloning cylinders. Workflow Timeline This protocol begins with a stable cell pool. For details, see our protocol...homogenized cell solution to 990 µL complete medium. Instead of transferring 0.125 µL, now transfer 100 times...

...you have a NanoDrop, follow the manufacturer's instructions for the spectrophotometer specific to your lab...

...Extraction Kit . Always follow the manufacturer's instructions. Note: It is usually important to determine ...

...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...

...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...

...the liquid and avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid...

...into polypeptide chains that make up the primary structure of proteins. Most proteins are then modified...systems. Insect expression systems Insect cells can also be used to produce complex eukaryotic proteins with...Researches can visualize proteins in vivo by tagging them with fluorescent proteins to study localization...localization or purify proteins to study their structure, interactions and functions. Proteins can also be purified... to manipulate DNA), or in medicine (e.g. insulin). Proteins, unlike DNA which can be relatively easily...are two types of insect expression systems; baculovirus infected and non-lytic insect cells. Baculovirus...genes integrated into the insect cell genome. Both of these types of insect expression systems can be ...

...       Insert-specific primers: Insert-specific primers are designed to anneal to an insert-specific... your insert or if the insert is in your plasmid Have to make a new primer pair for each insert Backbone-Specific...the insert, insert orientation, and the sequences of the junctions between the plasmid and insert DNA....negative clone without the insert. This type of primer pair can tell you if the insert is the correct size and...orientation of the insert. Orientation-specific primers: If you need information about insert orientation,...of the insert. One member of this type of primer pair anneals to a sequence flanking the insert and one...anneals to the insert. A simple way to create this type of primer pair is to mix-and-match insert-specific ...

...reporters as well as secretory proteins or proteins targeted to membrane sub-domains. It has also been used to... reporter mRNA, which contains the tethering sites for the fluorescent proteins and an inducible promoter...and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins that are bound...the nucleus (thanks to a NLS on these proteins). The RFP proteins, however, remain bound to the mRNA since...the Wyss Institute have extended the transactivating capabilities of CRISPR/dCas9-fusion proteins and have...engineered hook proteins fused to a streptavidin core that are able to retain reporter proteins fused to the...possible protein domains containing these properties, they generated monoclonal antibodies against them, fused...

... open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible... allow your insert to be placed into the backbone in the proper orientation. For instance, if you were...enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert can then ...used. Follow the manufacturer’s instructions.   2. Isolate Your Insert and Vector by Gel Purification ...separate your backbone away from any inserts cut out of it and your new insert from any overhangs cut off of ...these stains see the Bitesize Bio Blog and their associated manufacturers websites. These stains require...Your Insert into Your Vector In the ligation step, you mix your purified, cut backbone and insert in a...

...fused to two proteins, here proteins Y and Z, that are known to interact. When  proteins Y and Z interact...interacting proteins; nearby proteins are biotinylated when and where the two bait proteins interact, but...interact or from proteins that are just in the proximity of the bait proteins. Instead of pinpointing specific...restored, and nearby proteins are labeled with biotin. For labeling, BirA* fusion proteins are expressed in...activity is restored, and nearby proteins are labeled with biotin. When proteins Y and Z part ways, BirA* biotinylation...the biotin labeling remains even if the interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID...provides a candidate list of proteins that are part of the bait proteins’ interactome. Further characterization...

.... The Moffat laboratory maintains a website for the TKO library that contains up-to-date information and...(3) but its portability to other bacterial strains remains seriously limited, as prior optimizations are...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...optimization of industrial yeast strains. However, industrial yeast strains are more difficult to genetically...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...of RNA-binding proteins identified by editing Identifying targets of RNA-binding proteins (RBPs) has traditionally...