Addgene

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Addgene at the ASM Microbe 2016 Conference

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Blog Post

...wonderfully nerdy art for sale, and photos of the Agar Art competition entries were displayed in a gallery...
Choosing Your Perfect Empty Backbone

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Blog Post

...purify it – and you do not have a specific antibody against the protein available – you can always add an epitope...
10 Ways to Share Your Science!

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Blog Post

...science really works. 2) Read science writing in magazines or newspapers or other places online, and consider...
Plasmids 101: Antibiotic Resistance Genes

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Blog Post

...this post we'll focus primarily on antibiotics against Gram negative bacteria. In future posts, we'll ...
The Golden Stopwatch Award: Recognizing Facilitators of Research

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Blog Post

...Addgene can qualify. We also reward improvement against one’s own record Mention Addgene Master Agreements...
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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...peer-review comments will learn from them and propagate this culture further. Many thanks to our guest...
Fluorescent Biosensors for Measuring Autophagic Flux

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Blog Post

...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by...
Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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Blog Post

...Test multiple, small colonies After streaking LB agar plates, you may notice that there are both large...
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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Blog Post

...PBAD promoter. They created a bacterial lawn on an agar plate, put a mask over the plate containing the ...
10 Great Guest Posts We're Thankful For

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Blog Post

...is no longer available through Addgene. Thanks again to all of our fantastic depositors and guest writers...
Using Addgene's New Viral Service for Your Research

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...reagents. Virus distribution came up time and time again with many researchers lamenting the viral production...
In Vivo Biotinylation of Bacterial Fusion Proteins

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Blog Post

...EMD, AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves...
Summer SciComm Series: Master of SciComm

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Blog Post

...faculties into motion, such that a desire to once again write for fun eventually saw an English minor tacked...
Addgene Begins Distribution of Recombinant Antibodies

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Blog Post

...community demand. Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about ...
Addgene's Top 10 Blog Posts of 2015

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Blog Post

... by e-mailing us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog...
Don’t Wait To Launch Your Health Science Startup

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Blog Post

...company advancement. Be relentless The world is against you. As I mentioned, you start at a disadvantage...
Mentoring for Scientists: I Have a Mentor, What Now?

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Blog Post

...yourself. If you think you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate...
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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Blog Post

...cells within them work together or perhaps compete against each other. Getting two-color stimulation has been...
Reagent Repositories Are Speeding up Science During the Pandemic

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Blog Post

...research institution, has been able to rapidly propagate this strain, making it available to scientists...
What Good Citizenship Can Do for Reproducibility in Science

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Blog Post

...about issues with reproducibility, time and time again, the problem comes down to inadequate information...
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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Blog Post

...patient and rapidly test the variants in vitro against different drugs and dosages. Michal Bernach: Developing...
Visualizing Genomic Loci with CRISPR-Sirius

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Blog Post

...scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools were targeted to the FBN3...
K. phaffii: Rising to the Occasion

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Blog Post

...DOI: 10.3892/br.2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental...
Hot Plasmids - January 2023

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Blog Post

...second GFP antibody? Similarities: Generated against full length GFP and converted to recombinant form...
Summer SciComm Series: A PhD in Science Communication

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Blog Post

...school, and it’s oddly fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen...
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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...use this potential to make a difference not just against COVID-19, but also in other areas of scientific...
Networking Follow-up: I Met a Person. Now What?

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...send a long email. If you have more to say, email again or set up a meeting. If you really got along well...
Creating accessible biology activities in schools with BioBits

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Blog Post

...tiny amounts of reagents are, how complicated (and again, expensive) the different machines are, and how ...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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Blog Post

.... J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma...
Plasmids 101: NGS Quality Control for Pooled Libraries

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Blog Post

...plasmids in the library. Analyzing the reads is, again, seemingly straightforward – you need to count how...
DAP arrays for multiplex gene editing

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Blog Post

...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular dystrophies...
Components of CRISPR/Cas9

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Blog Post

...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The...
Addgene Welcomes John Doench to the Advisory Board

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Blog Post

...useful and what was used once and could be used again… is absolutely critical to [its] success and your...
Plasmids 101: Screening Strategies Used in Plasmid Cloning

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Blog Post

...resistance marker in the backbone. A selection on agar containing ampicillin will yield bacteria that have...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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...mCherry expression using FACs sorting, confirming yet again that sgRNA structure and genomic recognition sites...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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Blog Post

...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...
Early Career Researcher Toolbox: Social Media for Scientists

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...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...
Sharing is Caring: But How to Distribute Open Hardware?

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Blog Post

..., for example, can also be printed quickly in Madagascar. Platforms that allow for on-going creativity...
Antibodies 101: Isotypes

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Blog Post

...IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies. They...
GPCRs: How Do They Work and How Do We Study Them?

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Blog Post

....1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of ...

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